Influence of PLLA/PCL/HA scafffold fiber orientation on mechanical properties and osteoblast behavior

Autor: Nilza Ribeiro, Lilian de Siqueira, Eliandra de Sousa Trichês, Susana Sousa, Fernando J. Monteiro, Maria Helena Fernandes, Liliana Grenho, Cassilda Cunha-Reis, Maria B. A. Paredes
Přispěvatelé: Instituto de Investigação e Inovação em Saúde, Veritati - Repositório Institucional da Universidade Católica Portuguesa
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Scaffold
Materials science
Colony forming units
Scanning electron microscope
Modulus
Mechanical properties
02 engineering and technology
010402 general chemistry
01 natural sciences
PLLA
Article
chemistry.chemical_compound
Tissue engineering
Ultimate tensile strength
medicine
General Materials Science
Electrospraying
Electrospinning/electrospraying technique
Osteoblasts
Tissue engineering applications
Nanohydroxyapatite
Fourier transform infrared spectrometry
Bone-like biomaterial
Osteoblast
021001 nanoscience & nanotechnology
Osteoblast cell adhesion
Electrospinning
0104 chemical sciences
Random and aligned fibers
medicine.anatomical_structure
Nano-hydroxyapatite
chemistry
Aligned fibers
Polycaprolactone
Electrospinning/ electrospraying technique
PLLA/PCL/HA scaffolds
0210 nano-technology
Biomedical engineering
Zdroj: Materials
Volume 12
Issue 23
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 1996-1944
DOI: 10.3390/ma12233879
Popis: Scaffolds based on aligned and non-aligned poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibers obtained by electrospinning, associated to electrosprayed hydroxyapatite (HA) for tissue engineering applications were developed and their performance was compared in terms of their morphology and biological and mechanical behaviors. The morphological results assessed by scanning electron microscopy showed a mesh of PLLA/PCL fibers (random and perfectly aligned) associated with aggregates of nanophased HA. Fourier transform infrared spectrometry confirmed the homogeneity in the blends and the presence of nanoHA in the scaffold. As a result of fiber alignment a 15-fold increase in Young&rsquo
s Modulus and an 8-fold increase in tensile strength were observed when compared to non-aligned fibers. In PLLA/PCL/HA scaffolds, the introduction of nanoHA caused a remarkable improvement of the mechanical strength of this material acting as a reinforcement, enhancing the response of these constructs to tensile stress. In vitro testing was evaluated using osteoblast (MC3T3-E1) cells. The results showed that both fibrous scaffolds were able to support osteoblast cell adhesion and proliferation and that fiber alignment induced increased cellular metabolic activity. In addition, the adhesion and proliferation of Staphylococcus aureus were evaluated and a lower number of colony forming units (CFUs) was obtained in the scaffolds with aligned fibers.
Databáze: OpenAIRE
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