Profiles of antibiotic resistome and microbial community in groundwater of CKDu prevalence zones in Sri Lanka
| Autor: | Libing Zheng, Titus Cooray, Junya Zhang, S. K. Weragoda, Rohan Weerasooriya, Hui Zhong, Kbsn Jinadasa, Yuansong Wei |
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| Rok vydání: | 2020 |
| Předmět: |
Environmental Engineering
medicine.drug_class Health Toxicology and Mutagenesis Antibiotics 0211 other engineering and technologies Human pathogen 02 engineering and technology 010501 environmental sciences 01 natural sciences Environmental health medicine Prevalence Environmental Chemistry Humans Statistical analysis Renal Insufficiency Chronic Waste Management and Disposal Groundwater 0105 earth and related environmental sciences Sri Lanka 021110 strategic defence & security studies biology Microbiota Acinetobacter biology.organism_classification Pollution Resistome Anti-Bacterial Agents Microbial population biology Sri lanka |
| Zdroj: | Journal of hazardous materials. 403 |
| ISSN: | 1873-3336 |
| Popis: | The chronic kidney disease of unknown etiology (CKDu) prevalent in certain regions of Sri Lanka poses a serious threat to human health. Previous epidemiological studies focused on the search of causative agents for CKDu etiology from the viewpoint of groundwater composition, but how CKDu prevalence affected the groundwater microbial composition, especially the antibiotic resistome, has never been illuminated. This study investigated the response of microbial community and antibiotic resistome to CKDu prevalence in the groundwater through the high throughput sequencing and qPCR (HT-qPCR), respectively. Results showed that CKDu prevalence significantly influenced the distribution of antibiotic resistome and microbial community composition. The mexF dominated in all the groundwater samples and could be considered as an intrinsic ARG, and the β-lactamase cphA was specially enriched and closely associated with the antibiotics used for CKDu patients. The Acinetobacter was a potential human pathogen common in the groundwater of CKDu affected regions, while CKDu prevalence specially enriched the Aeromonas. Statistical analysis indicated that CKDu prevalence impacted antibiotic resistome through the microbial community as a whole, and MGEs contributed to the occurrence of mexF, while the enrichment of cphA could be attributed to the increase of Aeromonas. |
| Databáze: | OpenAIRE |
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