Prevalence of human herpesvirus-8 infection in HIV-positive patients with and without Kaposi's sarcoma in Hungary
| Autor: | Erika Szalai, János Hunyadi, Lajos Gergely, Györgyi Szládek, János Szlávik, Attila Juhász, Ravi Patel, Zsuzsanna Gerlei |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Genes Viral viruses HIV Infections Antibodies Viral Polymerase Chain Reaction law.invention Serology law Risk Factors Prevalence Immunology and Allergy Gammaherpesvirinae Child Fluorescent Antibody Technique Indirect Antigens Viral Polymerase chain reaction virus diseases Nuclear Proteins General Medicine Herpesviridae Infections Middle Aged Recombinant Proteins Infectious Diseases Herpesvirus 8 Human Female Microbiology (medical) Adult Adolescent Sexual Behavior Immunology Blotting Western Enzyme-Linked Immunosorbent Assay Biology Microbiology Virus Viral Proteins Antigen medicine Seroprevalence Humans Kaposi's sarcoma Sarcoma Kaposi Aged Hungary biology.organism_classification medicine.disease Virology Nested polymerase chain reaction |
| Zdroj: | FEMS immunology and medical microbiology. 43(2) |
| ISSN: | 0928-8244 |
| Popis: | Human herpesvirus-8 (HHV-8) infection of 130 Hungarian HIV-positive individuals with or without Kaposi's sarcoma was investigated from 158 serum and 122 peripheral blood samples using anti-latency-associated nuclear antigen (LANA) indirect immunofluorescence assay (IFA), recombinant orf65 and orfK8.1 antigen enzyme-linked immunosorbent assays (ELISAs), Western blot assays and orf26 specific nested polymerase chain reaction (PCR). The overall prevalence of HHV-8 infection was found to be 31.5% (41/130) among the Hungarian HIV-positive patients. This seroprevalence rate is 7–11-fold higher than that of healthy HIV-negative blood donors in Hungary. The highest prevalence of HHV-8 infection (36.1%, 35/97) was observed in homo- or bisexual patients. Similar to the serologic results, HHV-8 DNA was not always detectable in all serial samples previously shown to be positive for HHV-8 DNA. |
| Databáze: | OpenAIRE |
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