Distinct response of the hepatic transcriptome to Aflatoxin B1 induced hepatocellular carcinogenesis and resistance in rats
| Autor: | Jiejun Shi, Fenyong Sun, Xin Sun, Jiangtu He, Jing Lin, Cizhong Jiang, Chao Ou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Aflatoxin B1 Carcinoma Hepatocellular DNA repair Drug Resistance Apoptosis Biology medicine.disease_cause Article Transcriptome 03 medical and health sciences 0302 clinical medicine Gene expression medicine Cell Adhesion Animals Gene Regulatory Networks Gene Cell Proliferation Regulation of gene expression Genetics Multidisciplinary Sequence Analysis RNA Gene Expression Profiling Liver Neoplasms High-Throughput Nucleotide Sequencing Cell cycle medicine.disease Rats Gene Expression Regulation Neoplastic 030104 developmental biology Liver 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research RNA Long Noncoding Carcinogenesis |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Aflatoxin is a natural potent carcinogen and a major cause of liver cancer. However, the molecular mechanisms of hepatocellular carcinogenesis remain largely unexplored. In this study, we profiled global gene expression in liver tissues of rats that developed hepatocellular carcinoma (HCC) from aflatoxin B1 (AFB1) administration and those that were AFB1-resistant, as well as rats without AFB1 exposure as a control. AFB1 exposure resulted in extensive perturbation in gene expression with different functions in HCC and AFB1 resistance (AR) samples. The differentially expressed genes (DEGs) in HCC sample were enriched for cell proliferation, cell adhesion and vasculature development that largely contribute to carcinogenesis. Anti-apoptosis genes were up-regulated in HCC sample whereas apoptosis-induction genes were up-regulated in AR sample. AFB1 exposure also caused extensive alteration in expression level of lncRNAs. Among all the 4511 annotated lncRNAs, half of them were highly expressed only in HCC sample and up-regulated a group of protein-coding genes with cancer-related functions: apoptosis regulation, DNA repair and cell cycle. Intriguingly, these genes were down-regulated by lncRNAs highly expressed in AR sample. Collectively, apoptosis is the critical biological process for carcinogenesis in response to AFB1 exposure through changes in expression level of both protein-coding and lncRNA genes. |
| Databáze: | OpenAIRE |
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