On the Function of Trans-Splicing: No Evidence for Widespread Proteome Diversification in Trypanosomes
| Autor: | Cameron M Soulette, Scott William Roy, Oliver Oliverio |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
RNA
Spliced Leader Trypanosoma Spliceosome Letter Proteome Trypanosoma brucei brucei Trans-splicing Protozoan Proteins comparative genomics Biology 03 medical and health sciences 0302 clinical medicine trypanosome Molecular evolution Genetics Ribosome profiling Peptide Chain Initiation Translational Gene Ecology Evolution Behavior and Systematics 030304 developmental biology Comparative genomics trans-splicing 0303 health sciences molecular evolution bioinformatics Evolutionary biology RNA splicing spliced leader 030217 neurology & neurosurgery |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| Popis: | A long-standing mystery of genomic/transcriptomic structure involves spliced leader trans-splicing (SLTS), in which short RNA “tags” transcribed from a distinct genomic locus is added near the 5′ end of RNA transcripts by the spliceosome. SLTS has been observed in diverse eukaryotes in a phylogenetic pattern implying recurrent independent evolution. This striking convergence suggests important functions for SLTS, however no general novel function is known. Recent findings of frequent alternative SLTS (ALT-TS) suggest that ALT-TS could impart widespread functionality. Here, we tested the hypothesis that ALT-TS diversifies proteomes by comparing splicing patterns in orthologous genes between two deeply diverged trypanosome parasites. We also tested proteome diversification functions of ALT-TS by utilizing ribosome profiling sequence data. Finally, we investigated ALT-TS as a mechanism to regulate the expression of unproductive transcripts. Although our results indicate the functional importance of some cases of trans-splicing, we find no evidence for the hypothesis that proteome diversification is a general function of trans-splicing. |
| Databáze: | OpenAIRE |
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