'In vitro' effect of lipid peroxidation metabolites on elongation factor-2
| Autor: | Sandro Argüelles, Antonio Ayala, Alberto Machado |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Biophysics Acetaldehyde medicine.disease_cause Biochemistry Lipid peroxidation chemistry.chemical_compound Peptide Elongation Factor 2 tert-Butylhydroperoxide Malondialdehyde medicine Protein biosynthesis Benzene Derivatives Animals Rats Wistar Molecular Biology Aldehydes Chemistry Ketones In vitro Rats Elongation factor Oxidative Stress Liver Cumene hydroperoxide Toxicity Lipid Peroxidation Oxidative stress |
| Zdroj: | Biochimica et biophysica acta. 1760(3) |
| ISSN: | 0006-3002 |
| Popis: | Elongation Factor-2 (eEF-2) is the protein that catalyzes the translocation of the ribosome through mRNA. Not all oxidants affect eEF-2, which is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide and t-butyl hydroperoxide. Lipid peroxides constitute a potential hazard to living organisms because of their direct reactivity with a variety of biomolecules and the ability to decompose into free radicals and reactive aldehydes. In this "in vitro" study, we show the effect of three of these aldehydes on the levels of hepatic eEF-2. The results suggest that the toxicity associated with prooxidant-mediated hepatic lipid peroxidation on protein synthesis can originate from the interaction of the aldehydic end products of lipid peroxidation with eEF-2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|