Evaluation of the antibacterial and inhibitory activity of NorA and MepA efflux pumps from Staphylococcus aureus by diosgenin
| Autor: | Maria, do Socorro Costa, Ana Raquel Pereira, da Silva, Nara Juliana Santos, Araújo, José Maria Barbosa, Filho, Josean Fechine, Tavares, Thiago Sampaio, de Freitas, Francisco Nascimento, Pereira Junior, Erlânio Oliveira, de Sousa, Francisco Paulo Araujo, Maia, John Eversong Lucena, de Vasconcelos, Jacqueline Cosmo Andrade, Pinheiro, Henrique Douglas Melo, Coutinho |
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| Rok vydání: | 2022 |
| Předmět: |
Staphylococcus aureus
Microbial Sensitivity Tests General Medicine Diosgenin Saponins Staphylococcal Infections beta-Lactams General Biochemistry Genetics and Molecular Biology Anti-Bacterial Agents Aminoglycosides Bacterial Proteins Ethidium Escherichia coli Humans Ampicillin Gentamicins Multidrug Resistance-Associated Proteins General Pharmacology Toxicology and Pharmaceutics Fluoroquinolones Norfloxacin |
| Zdroj: | Life Sciences. 308:120978 |
| ISSN: | 0024-3205 |
| Popis: | The increase in bacterial resistance to available antibiotics has driven several researchers to search for new agents with therapeutic properties. Diosgenin is a naturally occurring steroidal saponin that has demonstrated several pharmacological properties. In the present study, we report the antimicrobial activity of diosgenin against the standard and multidrug-resistant bacteria of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, in addition to the efflux pump inhibitory activity against Staphylococcus aureus strains carrying NorA and MepA pumps. For this purpose, the broth microdilution method was used, from which the value of the Minimum Inhibitory Concentration (MIC) was obtained, and this was associated with subinhibitory concentration (MIC/8) with antibiotic of clinical use and ethidium bromide for strains carrier by efflux pump. Diosgenin showed antimicrobial activity for standard S. aureus bacteria and potentiating activity in association with gentamicin and ampicillin for P. aeruginosa multidrug-resistant bacteria, it also showed potentiation in association with norfloxacin against the E. coli strain and gentamicin against the S. aureus strain. Antimicrobial activity against efflux pump-bearing strains revealed that saponin did not interfere with the efflux pump mechanism or intervened antagonistically. Thus, saponin has shown to be very promising against bacterial resistance in association with aminoglycoside, fluoroquinolones and beta-lactam, however additional studies should be carried out to better elucidate the mechanism of action of diosgenin. |
| Databáze: | OpenAIRE |
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