Therapeutic prospects of hydroxytyrosol on experimentally induced diabetic testicular damage: potential interplay with AMPK expression
| Autor: | Amira E Alsemeh, Mai A. Samak, Samaa Salah Abd El-Fatah |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Histology Vimentin AMP-Activated Protein Kinases Testicular Diseases Antioxidants Pathology and Forensic Medicine Diabetes Mellitus Experimental Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Internal medicine Testis medicine Animals Rats Wistar biology Chemistry AMPK Cell Biology Phenylethyl Alcohol Sertoli cell Molecular medicine Rats Androgen receptor 030104 developmental biology Endocrinology medicine.anatomical_structure Apoptosis 8-Hydroxy-2'-Deoxyguanosine biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Cell and tissue research. 380(1) |
| ISSN: | 1432-0878 |
| Popis: | Male reproductive dysfunction represents one of the overlooked consequences of diabetes that still deserve more scientific attention. We designed this study to explore the therapeutic potential of hydroxytyrosol (HT) on diabetic testicular damage and to investigate its relationship with adenosine monophosphate-activated protein kinase (AMPK) expression. In this context, 30 adult male Wistar rats were utilized and subdivided into control, diabetic and HT-treated diabetic groups. Testicular sections were prepared for histopathological examination and immunohistochemical detection of 8-hydroxy-2'-deoxyguanosine, Sertoli cell vimentin, myoid cell α-SMA, androgen receptors and caspase-3. We also assessed oxidative enzymatic and lipid peroxidation biochemical profiles, sperm count, morphology and motility. Real-time PCR of AMPK expression in tissue homogenate was performed. We observed that HT restored testicular histopathological structure and significantly reduced oxidative DNA damage and the apoptotic index. The HT-treated group also exhibited significantly higher Sertoli cell vimentin, myoid cell α-SMA and androgen receptor immune expression than the diabetic group. A rescue of the oxidative enzymatic activity, lipid peroxidation profiles, sperm count, morphology and motility to control levels was also evident in the HT-treated group. Significant upregulation of AMPK mRNA expression in the HT-treated group clarified the role of AMPK as an underlying molecular interface of the ameliorative effects of HT. We concluded that HT exhibited tangible antioxidant and antiapoptotic impacts on the testicular cytomorphological and immunohistochemical effects of experimentally induced diabetes. Furthermore, AMPK has an impactful role in the molecular machinery of these effects. |
| Databáze: | OpenAIRE |
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