Combined toxic effects and DNA damage to two plant species exposed to binary metal mixtures (Cd/Pb)
| Autor: | J. Leclercq-Dransart, S. Dumez, Franck Vandenbulcke, A. Hayet, Anne Platel, Caroline Lanier, F. Bernard, Fabrice Nesslany, I. Devred, Annabelle Deram, Damien Cuny, Sébastien Lemière |
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| Přispěvatelé: | Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 (LGCgE), Université d'Artois (UA)-Université de Lille-Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-JUNIA (JUNIA), Université de Lille, Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut Lillois d'Ingénierie de la Santé (ILIS), Université de Lille, Droit et Santé, Université catholique de Lille (UCL)-Université catholique de Lille (UCL) |
| Rok vydání: | 2018 |
| Předmět: |
DNA damage
Endpoint Determination Health Toxicology and Mutagenesis 0211 other engineering and technologies chemistry.chemical_element 02 engineering and technology Brassica 010501 environmental sciences medicine.disease_cause 01 natural sciences Metal medicine Soil Pollutants 0105 earth and related environmental sciences 021110 strategic defence & security studies Cadmium biology Public Health Environmental and Occupational Health General Medicine biology.organism_classification Pollution Comet assay chemistry Lead 13. Climate action Bioaccumulation visual_art Environmental chemistry Toxicity [SDE]Environmental Sciences Trifolium repens visual_art.visual_art_medium Trifolium Comet Assay Genotoxicity DNA Damage |
| Zdroj: | Ecotoxicology and Environmental Safety Ecotoxicology and Environmental Safety, 2019, 167, pp.278--287. ⟨10.1016/j.ecoenv.2018.10.010⟩ |
| ISSN: | 1090-2414 0147-6513 |
| DOI: | 10.1016/j.ecoenv.2018.10.010⟩ |
| Popis: | ACL; Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity tests were carried out to assess the individual and combined toxic effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium repens. In addition to morphological parameters, this work also used comet assay to address endpoints in relation to genotoxicity. Bioaccumulation was measured to demonstrate the influence of the mixture on the concentrations of each metal in the plant. The statistical method reported by Ince et al. (1999) was used to evaluate the types of interaction between Cd and Pb in each treatment and concerning their combined effect. This study concludes that the combined effects of binary metal combinations of Cd/Pb on morphological parameters are most often additive, sometimes antagonistic and more rarely synergistic, thus extending the findings of previous publications on this subject. DNA damage analysis revealed concentration- and time-dependent interactions. Synergistic effects of mixed metals (more breaks than individually applied metals) are observed in T. repens after a short exposure. Antagonistic effects are statistically significant after 10 days-exposure, suggesting competition between metals. At 56 days, the rate of DNA damage observed in plants exposed to the Cd/Pb mixture was similar to that measured in plants exposed to lead only and was significantly lower than the rate of DNA damage induced by Cd. This supports the idea that there may be competition between metals and also strengthens the hypothesis that long-term reparation mechanisms may be implemented. Cd/Pb co-exposure does not significantly influence the bioaccumulation of each metal. It is nevertheless important to note that a statistically significant ‘interaction’ is not necessarily biologically relevant and should therefore be considered with caution when assessing heavy metals combined effects |
| Databáze: | OpenAIRE |
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