Autor: |
Ekaterina Makhneva, Dorota Sklenárová, Julian C. Brandmeier, Antonín Hlaváček, Hans H. Gorris, Petr Skládal, Zdeněk Farka |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Analytical chemistry. 94(47) |
ISSN: |
1520-6882 |
Popis: |
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels─horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)─UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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