The number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy

Autor: Mauro Masson Lerco, Célia Sperandeo Macedo, Daniela O. Pinheiro, César Tadeu Spadella, Reinaldo José da Silva
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
food intake
podocyte
Cell Count
Glomerulonephritis
Membranous
Diabetic nephropathy
chemistry.chemical_compound
Glomerulonephritis
Alloxan
Glomerular Basement Membrane
rat
Diabetic Nephropathies
glucose
education.field_of_study
Microscopy
Proteinuria
Podocytes
Glomerular basement membrane
Slit
fluid intake
Animal euthanasia
animal euthanasia
medicine.anatomical_structure
female
laboratory test
Slit diaphragm
Disease Progression
Female
medicine.symptom
medicine.medical_specialty
kidney
species comparison
animal experiment
Membranous
Electron
alloxan
animal tissue
Diabetes Mellitus
Experimental
body weight
Internal medicine
Diabetes mellitus
medicine
Diabetes Mellitus
Animals
controlled study
Rats
Wistar
education
glucose urine level
nonhuman
electron microscopy
business.industry
animal model
diabetic nephropathy
alloxan diabetes mellitus
medicine.disease
Rats
Microscopy
Electron
Endocrinology
glucose blood level
chemistry
glomerulus basement membrane
Surgery
proteinuria
business
cell membrane
Zdroj: Acta Cirúrgica Brasileira v.21 n.2 2006
Acta Cirúrgica Brasileira
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Popis: Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:49Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:29:35Z : No. of bitstreams: 1 2-s2.0-33645788562.pdf: 689363 bytes, checksum: 0fba4542825ef6faf0575fcac500771b (MD5) Made available in DSpace on 2014-05-27T11:21:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-03-01 Purpose: To determine the number of podocyte, slit diaphragms, slit diaphragm extensions and GBM thickness in diabetic nephropathy. Methods: Sixty Rattus Wistarof both sexes weighing 200-300g were divided in two experimental groups: normal group 10 animals, and alloxan diabetic rats - 50 animals. Alloxan was administered in a single IV dose of 42mg/kg body weight. Body weight, water and food intake, diuresis, and blood and urine glucose were determined in both groups before alloxan injection and two weeks, six and twelve months after alloxan injection. Proteinuria was measured at 12 months in both groups. After 12 months animals were sacrificed, and the right kidney processed for electron microscopy. Results: Clear clinical and laboratory signs of severe diabetes were seen, in all alloxan-diabetic rats at all follow-up times. Glomerular basement membrane (GBM) thickening, podocyte number, and slit diaphragm number and extension were determined. GBM of all diabetic rats was significantly thicker (median=0.29μm; semi-interquartile range=0.065μm) than in the normal rats (0.23μm; 0.035μm). Diabetic rat podocyte number (8; 1), slit diaphragm number (4; 1), and slit diaphragm extension (0.021μm; 0.00435μm) were significantly lower than in normal rats (11; 1) and (7; 1.5), and (0.031μm; 0.0058μm). Diabetic rat proteinuria (0.060mg/24h; 0.037mg/24h) was higher than in normal rats (0.00185mg/24h; 0.00055mg/24h). Conclusion: Experimental diabetes is associated with significant (p
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