Indomethacin: Plasma concentrations and protein binding in man
| Autor: | H. H. Lausen, Jens Aas Jansen, Eigill F. Hvidberg |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Time Factors Molar concentration Indomethacin Plasma protein binding chemistry.chemical_compound Methods Humans Drug Interactions Fluorometry Pharmacology (medical) Binding site Serum Albumin Pharmacology Chromatography Binding Sites Albumin General Medicine Hydrogen-Ion Concentration Fluorescence Salicylates Kinetics chemistry Sodium hydroxide Plasma concentration Dialysis Salicylic acid Protein Binding |
| Zdroj: | European Journal of Clinical Pharmacology. 4:119-124 |
| ISSN: | 1432-1041 0031-6970 |
| DOI: | 10.1007/bf00562508 |
| Popis: | The fluorometric method of Holt & Hawkins (1965) has been modified to permit determination of 50 ng indomethacin in 0.5 ml plasma, by measuring its fluorescence in a phosphate buffer at pH 11.6 instead of sodium hydroxide. The method has also been adapted to show the presence of salicylic acid and a column chromatographic method has been devised for its removal. The protein binding of indomethacin in human plasma was calculated to be about 90% from an association constant of 0.86×103 M−1 (M=molarity). The number of binding sites on albumin is about 15. The plasma levels of indomethacin in patients receiving continuous treatment with Indocid® were between 0.5 and 3 µg/ml during the 4–5 h immediately after the last dose of 25 mg. The disappearance of indomethacin from plasma appears to consist of a fast primary phase at plasma concentrations greater than 1 µg/ml (T 1/2 about 90 min.), and a slower secondary phase. |
| Databáze: | OpenAIRE |
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