Application of the central composite design to optimize the preparation of novel micelles of harmine
| Autor: | Xiao-Juan Hu, Xue-nong Zhang, Yong Jin, Qiao-Ling Zhu, Yang Liu, Peng Xia, Yong-Yan Bei, Xiao-feng Zhou, Wei-liang Chen, Zhi-qiang Yuan, Liang Zhang, Chun-ge Zhang, Ben-gang You |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Central composite design
Dispersity Biophysics Analytical chemistry Pharmaceutical Science Bioengineering Lactose Micelle Biomaterials response surface methodology Differential scanning calorimetry International Journal of Nanomedicine Drug Discovery Spectroscopy Fourier Transform Infrared chitosan derivate characterization Response surface methodology Particle Size central composite design self-assembled micelle Micelles Original Research chemistry.chemical_classification Chitosan Chromatography Models Statistical Calorimetry Differential Scanning Organic Chemistry Reproducibility of Results General Medicine Polymer Hydrogen-Ion Concentration Solvent Harmine chemistry Research Design Nanoparticles Particle size |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Yong-Yan Bei,1,* Xiao-Feng Zhou,2,3,* Ben-Gang You,1 Zhi-Qiang Yuan,1 Wei-Liang Chen,1 Peng Xia,1 Yang Liu,1 Yong Jin,4 Xiao-Juan Hu,1 Qiao-Ling Zhu,1 Chun-Ge Zhang,1 Xue-Nong Zhang,1 Liang Zhang5 1Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, People’s Republic of China; 2College of Radiological Medicine and Protection, Soochow University, Suzhou, People’s Republic of China; 3Changshu Hospital of Traditional Chinese Medicine, Changshu, People’s Republic of China; 4Invasive Technology Department, The Second Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China; 5Department of Biopharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou, People’s Republic of China*These authors contributed equally to the paperAbstract: Lactose–palmitoyl–trimethyl–chitosan (Lac-TPCS), a novel amphipathic self-assembled polymer, was synthesized for administration of insoluble drugs to reduce their adverse effects. The central composite design was used to study the preparation technique of harmine (HM)-loaded self-assembled micelles based on Lac-TPCS (Lac-TPCS/HM). Three preparation methods and single factors were screened, including solvent type, HM amount, hydration volume, and temperature. The optimal preparation technique was identified after investigating the influence of two independent factors, namely, HM amount and hydration volume, on four indexes, ie, encapsulation efficiency (EE), drug-loading amount (LD), particle size, and polydispersity index (PDI). Analysis of variance showed a high coefficient of determination of 0.916 to 0.994, thus ensuring a satisfactory adjustment of the predicted prescription. The maximum predicted values of the optimal prescription were 91.62%, 14.20%, 183.3 nm, and 0.214 for EE, LD, size, and PDI, respectively, when HM amount was 1.8 mg and hydration volume was 9.6 mL. HM-loaded micelles were successfully characterized by Fourier-transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, and a fluorescence-quenching experiment. Sustained release of Lac-TPCS/HM reached 65.3% in 72 hours at pH 7.4, while free HM released about 99.7% under the same conditions.Keywords: harmine, chitosan derivate, self-assembled micelle, central composite design, response surface methodology, characterization |
| Databáze: | OpenAIRE |
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