Characterization of Melan-A reactive memory CD8+ T cells in a healthy donor
| Autor: | Sarah Rowland-Jones, Gilbert Greub, Frederic Grosjean, Victor Appay, Nathalie Rufer, Serge Leyvraz, Theres Fagerberg, Roger H. Brookes, Verena Voelter, Clemencia Pinilla, Severine Reynard, Olivier Michelin, Philippe Guillaume, Pedro Romero |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cytotoxicity
Immunologic T cell Immunology Antigen presentation T-Cell Antigen Receptor Specificity CD8-Positive T-Lymphocytes Biology Cancer Vaccines Immunophenotyping Interleukin 21 Cross-Priming MART-1 Antigen Antigen Antigens Neoplasm medicine Humans Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Melanoma Tuberculosis Pulmonary Cells Cultured Antigens Bacterial CD40 Mycobacterium tuberculosis General Medicine Clone Cells Neoplasm Proteins medicine.anatomical_structure biology.protein Peptides Immunologic Memory |
| Zdroj: | International Immunology, vol. 20, no. 8, pp. 1087-1096 |
| Popis: | Melan-A specific CD8+ T cells are thought to play an important role against the development of melanoma. Their in vivo expansion is often observed with advanced disease. In recent years, low levels of Melan-A reactive CD8+ T cells have also been found in HLA-A2 healthy donors, but these cells harbor naive characteristics and are thought to be mostly cross-reactive for the Melan-A antigen. Here, we report on a large population of CD8+ T cells reactive for the Melan-A antigen, identified in one donor with no evidence of melanoma. Interestingly, this population is oligoclonal and displays a clear memory phenotype. However, a detailed study of these cells indicated that they are unlikely to be directly specific for melanoma, so that their in vivo expansion may have been driven by an exogenous antigen. Screening of a Melan-A cross-reactive peptide library suggested that these cells may be specific for an epitope derived from a Mycobacterium protein, which would provide a further example of CD8+ T cell cross-reactivity between a pathogen antigen and a tumor antigen. Finally, we discuss potential perspectives regarding the role of such cells in heterologous immunity, by influencing the balance between protective immunity and pathology, e.g. in the case of melanoma development. |
| Databáze: | OpenAIRE |
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