Damage criteria for cerebral cortex cells subjected to hyperthermia

Autor:	Rickard Liljemalm, Tobias Nyberg
Rok vydání:	2016
Předmět:	Hyperthermia Cancer Research medicine.medical_specialty Physiology Cell Survival 0206 medical engineering Finite Element Analysis Stimulation 02 engineering and technology Activation energy 01 natural sciences 010309 optics Physiology (medical) 0103 physical sciences medicine Animals Cell damage Cerebral Cortex Chemistry Pulse (signal processing) Lasers Temperature Pulse duration Hyperthermia Induced Models Theoretical medicine.disease 020601 biomedical engineering Surgery Cortex (botany) Rats medicine.anatomical_structure Cerebral cortex Biophysics
Zdroj:	International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group. 32(6)
ISSN:	1464-5157
Popis:	Temperatures above the normal physiological threshold may cause damage to cells and tissue. In this study, the response of a culture of dissociated cerebral cortex cells exposed to laser-induced temperature gradients was examined. The cellular response was evaluated using a fluorescent dye indicating metabolic activity. Furthermore, by using a finite element model of the heating during the pulsed laser application, threshold temperatures could be extracted for the cellular response at different laser pulse lengths. These threshold temperatures were used in an Arrhenius model to extract the kinetic parameters, i.e. the activation energy (Ea), and the frequency factor (Ac), for the system. A damage signal ratio was defined and calculated to 5% for the cells to increase their metabolism as a response to the heat. Furthermore, efficient stimulation with 20-ms long laser pulses did not evoke changes in metabolism. Thus, 20 ms could be a potential pulse length for functional stimulation of neural cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0e6d9742b89620034956e47ee9e1ec5 https://pubmed.ncbi.nlm.nih.gov/27269303 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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