Role of Lactobacillus pentosus Strain b240 and the Toll-Like Receptor 2 Axis in Peyer's Patch Dendritic Cell-Mediated Immunoglobulin A Enhancement
Autor: | Noriyuki Kohda, Ikutaro Sato, Yuji Suzuki, Jun Kunisawa, Masamichi Toba, Takao Saito, Yoshifumi Kotani, Hiroshi Kiyono |
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Rok vydání: | 2014 |
Předmět: |
Male
Immunoglobulin A B Cells medicine.medical_treatment lcsh:Medicine Biochemistry White Blood Cells Mice Peyer's Patches Animal Cells Interferon lcsh:Science Mice Inbred BALB C Toll-like receptor Immune System Proteins Multidisciplinary Animal Models medicine.anatomical_structure Cytokine Cytokines Anatomy Cellular Types Research Article medicine.drug Immune Cells Immunology Mouse Models Lactobacillus pentosus Biology Research and Analysis Methods Microbiology Antibodies Immunomodulation Immune Activation Model Organisms Immune system medicine Animals Nutrition Blood Cells Interleukin-6 lcsh:R Immunity Biology and Life Sciences Proteins Immunoregulation Peyer's patch Cell Biology Dendritic Cells Dendritic cell Molecular Development Molecular biology Acquired Immune System Toll-Like Receptor 2 Lactobacillus Immune System biology.protein lcsh:Q Clinical Immunology Digestive System Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 3, p e91857 (2014) |
ISSN: | 1932-6203 |
Popis: | Lactic acid bacteria are well known to possess immune-modulating effects, but the mechanisms underlying their modulation of the gut immune system are not fully understood. Here, we examined the localization of heat-killed Lactobacillus pentosus strain b240 (b240) in intestinal tissues and the effect of b240 on adaptive immune cascades in the gut. Histological analysis showed that b240 co-localized with dendritic cells (DCs) in the subepithelial dome region of Peyer's patches (PPs). In a PP cell culture system, b240 promoted the production of immunoglobulin A (IgA), interleukin (IL)-6, IL-10, interferon (IFN)-γ, and tumor necrosis factor, but not IL-4, IL-5, B-cell activating factors, IFN-α, IFN-β, and transforming growth factor-β1. The enhanced IgA production by b240 was attenuated by neutralizing IL-6, a potent IgA-enhancing cytokine. b240 stimulated DCs to produce an elevated amount of IL-6 in a Toll-like receptor (TLR) 2-, but not TLR4- or TLR9-dependent manner. Finally, we demonstrated that TLR2-mediated IL-6 production from PP DCs in response to b240 activated B cells to produce a large amount of IgA in a DC-B cell co-culture system. Our findings open up the possibility that the heat-killed form of Lactobacillus pentosus strain b240 can be used as a TLR2-mediated DC-activating biologic for enhancing IgA production in the intestine. |
Databáze: | OpenAIRE |
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