Role of Lactobacillus pentosus Strain b240 and the Toll-Like Receptor 2 Axis in Peyer's Patch Dendritic Cell-Mediated Immunoglobulin A Enhancement

Autor: Noriyuki Kohda, Ikutaro Sato, Yuji Suzuki, Jun Kunisawa, Masamichi Toba, Takao Saito, Yoshifumi Kotani, Hiroshi Kiyono
Rok vydání: 2014
Předmět:
Male
Immunoglobulin A
B Cells
medicine.medical_treatment
lcsh:Medicine
Biochemistry
White Blood Cells
Mice
Peyer's Patches
Animal Cells
Interferon
lcsh:Science
Mice
Inbred BALB C
Toll-like receptor
Immune System Proteins
Multidisciplinary
Animal Models
medicine.anatomical_structure
Cytokine
Cytokines
Anatomy
Cellular Types
Research Article
medicine.drug
Immune Cells
Immunology
Mouse Models
Lactobacillus pentosus
Biology
Research and Analysis Methods
Microbiology
Antibodies
Immunomodulation
Immune Activation
Model Organisms
Immune system
medicine
Animals
Nutrition
Blood Cells
Interleukin-6
lcsh:R
Immunity
Biology and Life Sciences
Proteins
Immunoregulation
Peyer's patch
Cell Biology
Dendritic Cells
Dendritic cell
Molecular Development
Molecular biology
Acquired Immune System
Toll-Like Receptor 2
Lactobacillus
Immune System
biology.protein
lcsh:Q
Clinical Immunology
Digestive System
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 3, p e91857 (2014)
ISSN: 1932-6203
Popis: Lactic acid bacteria are well known to possess immune-modulating effects, but the mechanisms underlying their modulation of the gut immune system are not fully understood. Here, we examined the localization of heat-killed Lactobacillus pentosus strain b240 (b240) in intestinal tissues and the effect of b240 on adaptive immune cascades in the gut. Histological analysis showed that b240 co-localized with dendritic cells (DCs) in the subepithelial dome region of Peyer's patches (PPs). In a PP cell culture system, b240 promoted the production of immunoglobulin A (IgA), interleukin (IL)-6, IL-10, interferon (IFN)-γ, and tumor necrosis factor, but not IL-4, IL-5, B-cell activating factors, IFN-α, IFN-β, and transforming growth factor-β1. The enhanced IgA production by b240 was attenuated by neutralizing IL-6, a potent IgA-enhancing cytokine. b240 stimulated DCs to produce an elevated amount of IL-6 in a Toll-like receptor (TLR) 2-, but not TLR4- or TLR9-dependent manner. Finally, we demonstrated that TLR2-mediated IL-6 production from PP DCs in response to b240 activated B cells to produce a large amount of IgA in a DC-B cell co-culture system. Our findings open up the possibility that the heat-killed form of Lactobacillus pentosus strain b240 can be used as a TLR2-mediated DC-activating biologic for enhancing IgA production in the intestine.
Databáze: OpenAIRE
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