Regeneration of a neoartery through a completely autologous acellular conduit in a minipig model: a pilot study
| Autor: | Song Li, Huimin Yan, Dong Wang, Sze Yue Wong, Nianguo Dong, Wen Zhao, Xuefeng Qiu, Kang Xu, Wang Tao |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology Swine lcsh:Medicine Pilot Projects Regenerative Medicine Cardiovascular Medical and Health Sciences Extracellular matrix Coronary artery bypass surgery 0302 clinical medicine Tissue engineering Models Medicine Miniature Assistive Technology Decellularization biology Tissue Scaffolds General Medicine Remodeling Carotid Arteries Heart Disease 030220 oncology & carcinogenesis Models Animal Swine Miniature Female Autologous medicine.medical_specialty Immunology Lumen (anatomy) Bioengineering Vascular graft Transplantation Autologous General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Blood Vessel Prosthesis Implantation Animals Regeneration Transplantation business.industry Heparin Animal Regeneration (biology) Research lcsh:R Vascular bypass Atherosclerosis Blood Vessel Prosthesis 030104 developmental biology biology.protein Autologous graft business Elastin |
| Zdroj: | Journal of translational medicine, vol 17, iss 1 Journal of Translational Medicine Journal of Translational Medicine, Vol 17, Iss 1, Pp 1-11 (2019) |
| Popis: | BackgroundVascular grafts are widely used as a treatment in coronary artery bypass surgery, hemodialysis, peripheral arterial bypass and congenital heart disease. Various types of synthetic and natural materials were experimented to produce tissue engineering vascular grafts. In this study, we investigated in vivo tissue engineering technology in miniature pigs to prepare decellularized autologous extracellular matrix-based grafts that could be used as vascular grafts for small-diameter vascular bypass surgery.MethodsAutologous tissue conduits (3.9mm in diameter) were fabricated by embedding Teflon tubings in the subcutaneous pocket of female miniature pigs (n = 8, body weight 25-30kg) for 4weeks. They were then decellularized by CHAPS decellularization solution. Heparin was covalently-linked to decellularized tissue conduits by Sulfo-NHS/EDC. We implanted these decellularized, completely autologous extracellular matrix-based grafts into the carotid arteries of miniature pigs, then sacrificed the pigs at 1 or 2months after implantation and evaluated the patency rate and explants histologically.ResultsAfter 1month, the patency rate was 100% (5/5) while the inner diameter of the grafts was 3.43 ± 0.05mm (n = 5). After 2months, the patency rate was 67% (2/3) while the inner diameter of the grafts was 2.32 ± 0.14mm (n = 3). Histological staining confirmed successful cell infiltration, and collagen and elastin deposition in 2-month samples. A monolayer of endothelial cells was observed along the inner lumen while smooth muscle cells were dominant in the graft wall.ConclusionA completely autologous acellular conduit with excellent performance in mechanical properties can be remodeled into a neoartery in a minipig model. This proof-of-concept study in the large animal model is very encouraging and indicates that this is a highly feasible idea worthy of further study in non-human primates before clinical translation. |
| Databáze: | OpenAIRE |
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