Iron Modulates Butyrate Production by a Child Gut Microbiota In Vitro
| Autor: | Christophe Lacroix, Rainer Follador, Van Thanh Pham, Lea Bircher, Michael B. Zimmermann, Christophe Chassard, Alexandra Dostal |
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| Rok vydání: | 2015 |
| Předmět: |
Pyruvate Synthase
Iron Butyrate Gut flora Gram-Positive Bacteria Microbiology chemistry.chemical_compound RNA Ribosomal 16S Virology Lactate dehydrogenase Gene expression Humans Roseburia intestinalis Microbiome Child Chromatography High Pressure Liquid 2. Zero hunger L-Lactate Dehydrogenase biology biology.organism_classification QR1-502 Gastrointestinal Microbiome Butyrates Biochemistry chemistry Fermentation Coenzyme A-Transferases Bacteria Research Article Hydrogen |
| Zdroj: | mBio, Vol 6, Iss 6 (2015) mBio mBio, 6 (6) |
| ISSN: | 2150-7511 2161-2129 |
| Popis: | The aim of this study was to investigate the effect of iron (Fe) availability on butyrate production in the complex bacterial ecosystem of the human gut. Hence, different Fe availabilities were mimicked in an in vitro colonic fermentation model (the polyfermenter intestinal model called PolyFermS) inoculated with immobilized gut microbiota from a child and in batch cultures of the butyrate producer Roseburia intestinalis. Shifts in the microbial community (16S rRNA sequencing and quantitative PCR), metabolic activity (high-performance liquid chromatography), and expression of genes involved in butyrate production were assessed. In the PolyFermS, moderate Fe deficiency resulted in a 1.4-fold increase in butyrate production and a 5-fold increase in butyryl-coenzyme A (CoA):acetate CoA-transferase gene expression, while very strong Fe deficiency significantly decreased butyrate concentrations and butyrate-producing bacteria compared with the results under normal Fe conditions. Batch cultures of R. intestinalis grown in a low-Fe environment preferentially produced lactate and had reduced butyrate and hydrogen production, in parallel with upregulation of the lactate dehydrogenase gene and downregulation of the pyruvate:ferredoxin-oxidoreductase gene. In contrast, under high-Fe conditions, R. intestinalis cultures showed enhanced butyrate and hydrogen production, along with increased expression of the corresponding genes, compared with the results under normal-Fe conditions. Our data reveal the strong regulatory effect of Fe on gut microbiota butyrate producers and on the concentrations of butyrate, which contributes to the maintenance of host gut health. IMPORTANCE Fe deficiency is one of the most common nutritional deficiencies worldwide and can be corrected by Fe supplementation. In this in vitro study, we show that environmental Fe concentrations in a continuous gut fermentation model closely mimicking a child’s gut microbiota strongly affect the composition of the gut microbiome and its metabolic activity, particularly butyrate production. The differential expression of genes involved in the butyrate production pathway under different Fe conditions and the enzyme cofactor role of Fe explain the observed modulation of butyrate production. Our data reveal that the level of dietary Fe reaching the colon affects the microbiome, and its essential function of providing the host with beneficial butyrate. |
| Databáze: | OpenAIRE |
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