Immunoneutralization of TGFβ1 Improves Skeletal Muscle Regeneration: Effects on Myoblast Differentiation and Glycosaminoglycan Content
| Autor: | Malgorzata Zimowska, Jerzy Moraczewski, P. Dragun, A. Oberbek, A. Duchesnay, Isabelle Martelly |
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| Rok vydání: | 2009 |
| Předmět: |
Article Subject
education behavioral disciplines and activities Glycosaminoglycan Fibrosis mental disorders medicine Myocyte lcsh:QH573-671 Receptor Soleus muscle lcsh:Cytology Chemistry musculoskeletal neural and ocular physiology Regeneration (biology) Skeletal muscle Cell Biology musculoskeletal system medicine.disease Cell biology medicine.anatomical_structure Immunology In vitro growth tissues psychological phenomena and processes Research Article |
| Zdroj: | International Journal of Cell Biology, Vol 2009 (2009) International Journal of Cell Biology |
| ISSN: | 1687-8884 1687-8876 |
| DOI: | 10.1155/2009/659372 |
| Popis: | When injured by crushing, the repair of the slow-twitch soleus rat muscle, unlike the fast-twitch EDL, is associated with fibrosis. As TGFβ1, whose activity can be controlled by glycosaminoglycans (GAG), plays a major role in fibrosis, we hypothesized that levels of TGFβ1 and GAG contents could account for this differential quality of regeneration. Here we show that the regeneration of the soleus was accompanied by elevated and more sustained TGFβ1 level than in the EDL. Neutralization of TGFβ1 effects by antibodies to TGFβ1 or its receptor TGFβ-R1 improved muscle repair, especially of the soleus muscle, increased in vitro growth of myoblasts, and accelerated their differentiation. These processes were accompanied by alterations of GAG contents. These results indicate that the control of TGFβ1 activity is important to improve regeneration of injured muscle and accelerate myoblast differentiation, in part through changes in GAG composition of muscle cell environment. |
| Databáze: | OpenAIRE |
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