Antibody-mediated bacterial clearance from the lower respiratory tract of mice requires complement component C3
| Autor: | Eric T. Harvill, Lakshmi Gopinathan, Mylisa R. Pilione, Mary J. Kennett, Elizabeth J. Pishko, Girish S. Kirimanjeswara |
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| Rok vydání: | 2004 |
| Předmět: |
Vaccines
Bordetella bronchiseptica biology Phagocytosis Immunology Macrophage-1 Antigen chemical and pharmacologic phenomena Context (language use) Complement C3 Pneumonia biology.organism_classification Microbiology Mice Inbred C57BL Antibody opsonization Bordetella Mice Immunity Macrophage-1 antigen biology.protein Animals Immunology and Allergy Antibody Lung |
| Zdroj: | European Journal of Immunology. 34:184-193 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | To assess the contribution of complement to respiratory immunity in the context of a natural bacterial infection, we used mice genetically deficient in complement components and the murine pathogen Bordetella bronchiseptica. Complement component C3 was not required for the control of bacterial infection or for the generation of infection-induced protective immunity. However, C3-deficient (C3(-/-)) mice were severely defective, compared to wild type, in vaccine-induced protective immunity. Adoptively transferred immune serum from convalescent wild-type or C3(-/-) animals rapidly cleared B. bronchiseptica from the lungs of wild-type mice but did not affect its growth in C3(-/-) mice, indicating that the defect is not in the generation of protective immunity, but in its function. Immune serum was effective in C5-deficient mice but had little effect in the lungs of mice lacking either Fcgamma receptors (FcgammaR) or CR3, suggesting bacterial clearance is not via direct complement-mediated lysis. Together, these data indicate that complement is required for antibody-mediated clearance of Bordetella and suggest the mechanism involves C3 opsonization of bacteria for phagocytosis that is both CR3- and FcgammaR-dependent. |
| Databáze: | OpenAIRE |
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