Isoflavonoids from Astragalus mongholicus protect PC12 cells from toxicity induced by L-glutamate
| Autor: | Chaoliang Wei, Yong-Ming Bao, Yanlong Duan, Li-Jia An, Dehong Yu |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Antioxidant
DPPH Cell Survival medicine.medical_treatment Drug Evaluation Preclinical Glutamic Acid Tetrazolium Salts PC12 Cells Plant Roots Superoxide dismutase chemistry.chemical_compound Glucoside Picrates Drug Discovery medicine Formononetin Animals Ononin Pharmacology chemistry.chemical_classification Neurons Glutathione Peroxidase Formazans biology Dose-Response Relationship Drug Ethanol L-Lactate Dehydrogenase Plant Extracts Superoxide Dismutase Glutathione peroxidase Biphenyl Compounds Astragalus Plant Free Radical Scavengers Glutathione Isoflavones Rats Calycosin Hydrazines Biochemistry chemistry biology.protein Drugs Chinese Herbal |
| Zdroj: | Journal of ethnopharmacology. 98(1-2) |
| ISSN: | 0378-8741 |
| Popis: | lsoflavonoids, formononetin, 9,10-dimethoxypterocarpan 3- O -β- d -glucoside, ononin, calycosin 7- O -glc and calycosin, were isolated from the roots of Astragalus mongholicus Bunge (Leguminosae). The neuroprotective roles and direct antioxidant effects of these isoflavonoids were investigated by using PC12 cell model and DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Formononetin, ononin and calycosin were found inhibiting glutamate-induced cell injury, with an estimated 50% effective concentration (EC 50 ) of 0.027 μg/ml, 0.047 μg/ml and 0.031 μg/ml, respectively. Pretreatment with them increased the activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and prevented the release of lactate dehydrogenase (LDH) in glutamate-injured PC12 cells. On the other hand, calycosin 7- O -glc and calycosin showed more scavenging activity to DPPH radicals than formononetin in the cell-free system. The inconsistency between the neuroprotective capabilities of isoflavonoids and their directly scavenging activity to DPPH radicals indicated that formononetin, ononin and calycosin probably depended on increasing endogenous antioxidant and stabilizing the cells’ membrane structures to inhibit the cell damage induced by glutamate. |
| Databáze: | OpenAIRE |
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