Calcium regulation of colonic crypt cell kinetics: Evidence for a direct effect in mice
| Autor: | C Nobre-Leitão, Maria A. Ferra, P Fidalgo, António Gouveia-Oliveira, Marília Cravo, Paula Chaves, F C Mira |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Colon Ratón chemistry.chemical_element Mice Inbred Strains Calcium Malignant transformation Mice chemistry.chemical_compound Internal medicine medicine Dimethylhydrazine Animals Diet Fat-Restricted Dimethylhydrazines Calcium metabolism Hepatology Chemistry Cell growth Gastroenterology 1 2-Dimethylhydrazine Cell Transformation Neoplastic Endocrinology Carcinogens Female Colorectal Neoplasms Cell Division Bromodeoxyuridine |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0016-5085 |
| DOI: | 10.1016/0016-5085(95)90338-0 |
| Popis: | Background & Aims: Oral calcium supplementation is believed to decrease colonic hyperproliferation through neutralization of fatty acids and bile acids. In the present study, the effect of oral calcium, given with low-fat diets, in the early stages of colorectal carcinogenesis is evaluated. Methods: In experiment A, mice received normal or low-calcium diets and were killed at 25 weeks. In experiment B, mice were fed the same diets but were submitted to six weekly injections of dimethylhydrazine and were killed at 10, 16, and 21 weeks. Cell proliferation was evaluated using bromodeoxyuridine immunohistochemistry. Results: In experiment A, mice fed low-calcium diets showed a significant upward shift of the proliferative compartment ( P = 0.04) (phase 2 defect) in the absence of hyperproliferation. In experiment B, besides a phase 2 defect, dimethylhydrazine-induced hyperproliferation was also significantly enhanced in animals fed low-calcium diets (phase 1 defect) as shown by an increased number of labeled cells per column and total labeling index ( P = 0.01). Conclusions: Low-calcium diets induce an upward shift of the main proliferative compartment, which reflects an increased risk for malignant transformation. This effect was observed with a low-fat diet, suggesting a direct mechanism, rather than the usual indirect one, documented with high-fat diets. |
| Databáze: | OpenAIRE |
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