Identification of cow's milk in 'buffalo' cheese by duplex Polymerase Chain Reaction
| Autor: | Sergio Rosati, A. Anastasio, R. M. Turi, Maria Teresa Bottero, Tiziana Civera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Buffaloes
Molecular Sequence Data Microbiology Polymerase Chain Reaction law.invention Qualitative analysis Species Specificity law Cheese Food Labeling Animals Polymerase Polymerase chain reaction biology Base Sequence Gene Amplification food and beverages Molecular biology Duplex pcr Milk Duplex (building) Retail trade biology.protein Cattle Mozzarella cheese Sequence Alignment Control methods Food Science |
| Zdroj: | Scopus-Elsevier |
| Popis: | A duplex polymerase chain reaction (PCR) was developed to identify the milk of bovine and buffalo species in cheese products, particularly in mozzarella cheese, a typical Italian cheese made from buffalo's milk. Two sets of primers were designed on the basis of the alignment of the sequence codifying mitochondrial cyt h available in the GenBank database. The primers proved to be species-specific, giving rise to 279-bp (bovine) and 192-bp (buffalo) amplified fragments. Since the amplification conditions for bovine and buffalo primers were identical, a duplex PCR was successfully applied to identify the two species in a single reaction step. This technique, when used to test cheese products from the retail trade, allowed the detection of partial or even total substitution of cow's milk for buffalo's milk, in some cases in samples of cheese misleadingly labeled pure buffalo mozzarella. Le sujet de cette etude est de developper une methode de reaction en chaine par polymerase (PCR) duplex, capable d'identifier et de differencier l'ADN bovin et l'ADN de buffle dans une reaction PCR unique. Cette methode est utilisee pour detecter la presence de lait de vache dans des fromages etiquetes pur buffle. L'evaluation des origines des laits utilises est tres importante en Italie ou une mozarella au lait de buffle est protegee par une appellation d'origine. |
| Databáze: | OpenAIRE |
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