Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties

Autor: Jannet Kammoun, Saoussen Hammami, Hassiba Chahdoura, Hanen Louati, Guido Flamini, Boulbaba Selmi, Malek Besbes Hlila, Lotfi Achour, Habib Mosbah
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Antioxidant
medicine.medical_treatment
Chemical composition
01 natural sciences
Antioxidants
Gas Chromatography-Mass Spectrometry
law.invention
Fungal Proteins
chemistry.chemical_compound
0404 agricultural biotechnology
Antioxidant activity
Rhaponticum acaule essential oil
law
Oils
Volatile
medicine
Glycoside Hydrolase Inhibitors
Xanthine oxidase
Enzyme Inhibitors
Hypoxanthine
Essential oil
ABTS
010405 organic chemistry
alpha-Glucosidases
04 agricultural and veterinary sciences
General Medicine
lcsh:Other systems of medicine
Xanthine
lcsh:RZ201-999
040401 food science
Pancreatic lipase inhibition
Leuzea
0104 chemical sciences
α-glucosidase
Antioxidant activity
Chemical composition
Pancreatic lipase inhibition
Rhaponticum acaule essential oil
Xanthine oxidase
Kinetics
Complementary and alternative medicine
chemistry
Biochemistry
Uric acid
α-glucosidase
Aspergillus niger
Lipid digestion
Research Article
Zdroj: BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-12 (2018)
BMC Complementary and Alternative Medicine
ISSN: 1472-6882
Popis: Background α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention. Methods The flowers essential oil of Rhaponticum acaule (L) DC (R. acaule) was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activities of R. acaule essential oil (RaEO) were also determined using 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power, phosphomolybdenum, and DNA nicking assays. The inhibitory power of RaEO against α-glucosidase, xanthine oxidase and pancreatic lipase was evaluated. Enzyme kinetic studies using Michaelis-Menten and the derived Lineweaver-Burk (LB) plots were performed to understand the possible mechanism of inhibition exercised by the components of this essential oil. Results The result revealed the presence of 26 compounds (97.4%). The main constituents include germacrene D (49.2%), methyl eugenol (8.3%), (E)-β-ionone (6.2%), β-caryophyllene (5.7%), (E,E)-α-farnesene (4.2%), bicyclogermacrene (4.1%) and (Z)-α-bisabolene (3.7%). The kinetic inhibition study showed that the essential oil demonstrated a strong α-glucosidase inhibiton and it was a mixed inhibitor. On the other hand, our results evidenced that this oil exhibited important xanthine oxidase inhibitory effect, behaving as a non-competitive inhibitor. The essential oil inhibited the turkey pancreatic lipase, with maximum inhibition of 80% achieved at 2 mg/mL. Furthermore, the inhibition of turkey pancreatic lipase by RaEO was an irreversible one. Conclusion The results revealed that the RaEO is a new promising potential source of antioxidant compounds, endowed with good practical applications for human health.
Databáze: OpenAIRE
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