Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip
| Autor: | Arnaud Nicolas, Carola M. E. Ammerlaan, Linda Gijzen, Marianne K. Vormann, Paul Vulto, Maarten B. Rookmaaker, Frans Schutgens, Dorota Kurek, Hans Clevers, Fjodor A. Yousef Yengej, Henriëtte L. Lanz, Marianne C. Verhaar |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Adolescent Microfluidics Kidney Tubules/growth & development Biology Cell Fractionation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Young Adult 0302 clinical medicine Membrane Transport Proteins/metabolism In vivo Lab-On-A-Chip Devices medicine 80 and over Electric Impedance Animals Humans Fluorescent Dyes/chemistry Child Preschool 030304 developmental biology Aged Aged 80 and over 0303 health sciences Kidney urogenital system Mesenchymal stem cell Infant Tissue Culture Techniques/methods Middle Aged In vitro Cell biology Rats Perfusion medicine.anatomical_structure Child Preschool Renal physiology Female Cell fractionation 030217 neurology & neurosurgery Function (biology) Organoids/growth & development |
| Zdroj: | Nature Protocols, 16(4), 2023-2050. Nature Publishing Group |
| ISSN: | 1754-2189 |
| DOI: | 10.1038/s41596-020-00479-w |
| Popis: | Advanced in vitro kidney models are of great importance to the study of renal physiology and disease. Kidney tubuloids can be established from primary cells derived from adult kidney tissue or urine. Tubuloids are three-dimensional multicellular structures that recapitulate tubular function and have been used to study infectious, malignant, metabolic, and genetic diseases. For tubuloids to more closely represent the in vivo kidney, they can be integrated into an organ-on-a-chip system that has a more physiological tubular architecture and allows flow and interaction with vasculature or epithelial and mesenchymal cells from other organs. Here, we describe a detailed protocol for establishing tubuloid cultures from tissue and urine (1-3 weeks), as well as for generating and characterizing tubuloid cell-derived three-dimensional tubular structures in a perfused microfluidic multi-chip platform (7 d). The combination of the two systems yields a powerful in vitro tool that better recapitulates the complexity of the kidney tubule with donor-specific properties. |
| Databáze: | OpenAIRE |
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