Bordetella pertussis PCR: simultaneous targeting of signature sequences
| Autor: | Lynn O. Stapp, Brian P. Ingersoll, Patrick M. Abe, Peter W. Monsaas, Christina J. Lemoine, Treva Tsosie, Xuan Qin, David K. Turgeon |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Bordetella pertussis Adolescent Whooping Cough Biology Pertussis toxin Polymerase Chain Reaction Sensitivity and Specificity law.invention Immunoenzyme Techniques Species Specificity law Nasopharynx Multiplex polymerase chain reaction medicine Humans Virulence Factors Bordetella Insertion sequence Child Promoter Regions Genetic Direct fluorescent antibody Whooping cough Polymerase chain reaction Base Sequence Infant General Medicine Amplicon medicine.disease biology.organism_classification Virology Molecular biology Infectious Diseases Pertussis Toxin Child Preschool DNA Transposable Elements |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 43:269-275 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/s0732-8893(02)00405-4 |
| Popis: | The absence of analytical controls for polymerase chain reaction (PCR)-based diagnostic tests for Bordetella pertussis limits their clinical utility. In this study, multiplex PCR simultaneously targeted two specific Bordetella pertussis sequences, the chromosomal repeated insertion sequence IS481 (IS) and the pertussis toxin promoter region (PT). A multi-target hybridization-EIA (Hyb-EIA) method in a 96-well microtiter-plate format was used to detect amplicons. Forty-seven (15%) of the 318 nasopharygeal specimens tested positive for at least one DNA target of B. pertussis by PCR, including the 10 known positive samples by culture and/or direct fluorescent antibody (DFA). Forty-six of the 47 PCR positive samples were considered positive for B. pertussis using the consensus interpretation criteria. Simultaneous detection of multiple chromosomal regions may identify false-positive and -negative results due to analytical variations or potential sequence polymorphism, and uncover a wider range of pathogenic strains. |
| Databáze: | OpenAIRE |
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