Nanoparticles Based on Linear and Star-Shaped Poly(Ethylene Glycol)-Poly(ε-Caprolactone) Copolymers for the Delivery of Antitubulin Drug
| Autor: | Mahmoud E. S. Soliman, Giulia Bonacucina, Omaima A. Sammour, Lisbeth Illum, Giovanni Filippo Palmieri, Abdelhameed A. El Shamy, Karim S. Shalaby, Marco Cespi, Luca Casettari |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Drug
Noscapine Cell Survival media_common.quotation_subject Polyesters Pharmaceutical Science Nanoparticle pH dependent release passive targeting polyethylene glycol-co-poly(ε-caprolactone) (PEG-co-PCL) polymeric nanoparticles 02 engineering and technology Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Cell Line Tumor Polymer chemistry medicine Copolymer Animals Humans Pharmacology (medical) Solubility Particle Size Rats Wistar media_common Pharmacology Dose-Response Relationship Drug Organic Chemistry technology industry and agriculture Polyethylene 021001 nanoscience & nanotechnology Polymeric nanoparticles Combinatorial chemistry Rats chemistry 030220 oncology & carcinogenesis MCF-7 Cells Molecular Medicine Nanoparticles Female 0210 nano-technology Caprolactone Biotechnology medicine.drug |
| Popis: | Biodegradable polymeric nanoparticles of different architectures based on polyethylene glycol-co-poly(ε-caprolactone) block copolymers have been loaded with noscapine (NOS) to study their effect on its anticancer activity. It was intended to use solubility of NOS in an acidic environment and ability of the nanoparticles to passively target drugs into cancer tissue to modify the NOS pharmacokinetic properties and reduce the requirement for frequent injections.Linear and star-shaped copolymers were synthetized and used to formulate NOS loaded nanoparticles. Cytotoxicity was performed using a sulforhodamine B method on MCF-7 cells, while biocompatibility was determined on rats followed by hematological and histopathological investigations.Formulae with the smallest particle sizes and adequate entrapment efficiency revealed that NOS loaded nanoparticles showed higher extent of release at pH 4.5. Colloidal stability suggested that nanoparticles would be stable in blood when injected into the systemic circulation. Loaded nanoparticles had IC50 values lower than free drug. Hematological and histopathological studies showed no difference between treated and control groups. Pharmacokinetic analysis revealed that formulation P1 had a prolonged half-life and better bioavailability compared to drug solution.Formulation of NOS into biodegradable polymeric nanoparticles has increased its efficacy and residence on cancer cells while passively avoiding normal body tissues. Graphical Abstract ᅟ. |
| Databáze: | OpenAIRE |
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