Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality
| Autor: | Timothy J. Nelson, Frank J. Secreto, Alyson J. Smith, Bonnie K. Arendt, Sybil C. L. Hrstka, Ester Perales-Clemente, Emma B. Brandt, Elizabeth S. Bruinsma, Xing Li, Gresin Hawse, Saji Oommen, Dennis A. Wigle |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells medicine.drug_class Topoisomerase Inhibitors Cell Induced Pluripotent Stem Cells Clone (cell biology) Biology Colony-Forming Units Assay 03 medical and health sciences Technological Advancements Translational Research Articles and Reviews Quantification medicine Hypersensitivity Humans Stem Cell Niche Induced pluripotent stem cell Etoposide Embryonic Stem Cells Cells Cultured Clinical Trials as Topic Functional RNA Cell Biology General Medicine medicine.disease Molecular biology Cell biology 030104 developmental biology medicine.anatomical_structure Teratoma Stem cell Transcriptome Topoisomerase inhibitor Developmental Biology medicine.drug |
| Zdroj: | Stem Cells Translational Medicine |
| ISSN: | 2157-6580 2157-6564 |
| Popis: | Human induced pluripotent stem cells (hiPSC) hold great promise in diagnostic and therapeutic applications. However, translation of hiPSC technology depends upon a means of assessing hiPSC quality that is quantitative, high-throughput, and can decipher malignant teratocarcinoma clones from normal cell lines. These attributes are lacking in current approaches such as detection of cell surface makers, RNA profiling, and/or teratoma formation assays. The latter remains the gold standard for assessing clone quality in hiPSCs, but is expensive, time-consuming, and incompatible with high-throughput platforms. Herein, we describe a novel method for determining hiPSC quality that exploits pluripotent cells’ documented hypersensitivity to the topoisomerase inhibitor etoposide (CAS No. 33419-42-0). Based on a study of 115 unique hiPSC clones, we established that a half maximal effective concentration (EC50) value of |
| Databáze: | OpenAIRE |
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