Protective effects of 5-aminolevulinic acid on heat stress in bovine mammary epithelial cells
| Autor: | Shinichi Yonekura, Yoko Noguchi, Shin Taniguchi, Aminul Islam |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Heat Stress
Bovine Mammary Epithelial Cells Physiology medicine.disease_cause Article Downregulation and upregulation Genetics medicine Viability assay 5-Aminolevulinic Acid General Veterinary Chemistry Endoplasmic reticulum Endoplasmic Reticulum Stress Molecular biology Cytoprotection Oxidative Stress Real-time polymerase chain reaction QL1-991 Apoptosis Animal Reproduction and Physiology Unfolded protein response Animal Science and Zoology Zoology Oxidative stress Food Science |
| Zdroj: | Animal Bioscience Animal Bioscience, Vol 34, Iss 6, Pp 1006-1013 (2021) |
| ISSN: | 2765-0235 2765-0189 |
| DOI: | 10.5713/ajas.20.0349 |
| Popis: | Objective: Cells have increased susceptibility to activation of apoptosis when suffering heat stress (HS). An effective supplementation strategy to mimic heat-induced apoptosis of bovine mammary epithelial cells (MECs) is necessary to maintain optimal milk production. This study aimed to investigate possible protective effects of the anti-apoptotic activity of 5-aminolevulinic acid (5-ALA) against HS-induced damage of bovine MECs.Methods: Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 μM for 24 h followed by HS (42.5°C for 24 h and 48 h). Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress genes.Results: We found that 5-ALA induces cytoprotection via inhibition of apoptosis markers after HS-induced damage. Pretreatment of bovine MECs with 5-ALA resulted in dramatic upregulation of mRNA for nuclear factor erythroid-derived 2-like factor 2, heme oxygenase-1, and NAD(P)H quinone oxidoreductase 1, all of which are antioxidant stress genes. Moreover, 5-ALA pretreatment significantly suppressed HS-induced ER stress-associated markers, glucose-regulated protein 78, and C/EBP homologous protein expression levels.Conclusion: 5-ALA can ameliorate the ER stress in heat stressed bovine MEC via enhancing the expression of antioxidant gene. |
| Databáze: | OpenAIRE |
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