Aortic eNOS expression and phosphorylation in Apo-E knockout mice: Differing effects of rapamycin and simvastatin
Autor: | Glenn C. Hunter, Joseph J. Naoum, Qian Guo, Kenneth J. Woodside, Wei Song, Ligia M. Belalcazar, Shu Zhang |
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Rok vydání: | 2004 |
Předmět: |
Male
Apolipoprotein E Simvastatin medicine.medical_specialty Nitric Oxide Synthase Type III Caveolin 1 Nitric Oxide Synthase Type II Caveolins Nitric oxide Mice chemistry.chemical_compound Apolipoproteins E Enos Internal medicine medicine Animals Phosphorylation Aorta Antibacterial agent Mice Knockout Sirolimus Triglyceride biology Endothelial Cells biology.organism_classification Immunohistochemistry Cholesterol Endocrinology chemistry Enzyme inhibitor Knockout mouse biology.protein Surgery Nitric Oxide Synthase medicine.drug |
Zdroj: | Surgery. 136:323-328 |
ISSN: | 0039-6060 |
DOI: | 10.1016/j.surg.2004.05.007 |
Popis: | Background. The inhibition of nitric oxide (NO) by hypercholesterolemia may be mediated, in part, by interactions with caveolin-1 (Cav-1). Because of the facililatory effects of statins on endothelial function and the adverse effect of rapamycin (RAPA) on plasma lipids, we compared the effects of simvastatin (SMV) and RAPA on endothelial NO synthase (eNOS) and Cav-1 protein expression and phosphorylation in the aortas of apolipoprotein E (Apo-E) knockout (-/-) mice. Methods. Apo-E -/- mice (n = 38) fed a high-cholesterol diet were given SMV (100 mg/kg/day po), RAPA (3 mg/kg/day ip), or no treatment for 10 weeks. Blood was drawn for serum lipid analysis, and protein was extracted for Western immunoblotting. Selected aortic specimens from 2 animals in each group were examined by histology and immunohistochemistry. The data are expressed as the mean ± SEM and compared by the Student t test and ANOVA. Significance was established as P |
Databáze: | OpenAIRE |
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