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Aortic eNOS expression and phosphorylation in Apo-E knockout mice: Differing effects of rapamycin and simvastatin

Autor: Glenn C. Hunter, Joseph J. Naoum, Qian Guo, Kenneth J. Woodside, Wei Song, Ligia M. Belalcazar, Shu Zhang
Rok vydání: 2004
Předmět:
Zdroj: Surgery. 136:323-328
ISSN: 0039-6060
DOI: 10.1016/j.surg.2004.05.007
Popis: Background. The inhibition of nitric oxide (NO) by hypercholesterolemia may be mediated, in part, by interactions with caveolin-1 (Cav-1). Because of the facililatory effects of statins on endothelial function and the adverse effect of rapamycin (RAPA) on plasma lipids, we compared the effects of simvastatin (SMV) and RAPA on endothelial NO synthase (eNOS) and Cav-1 protein expression and phosphorylation in the aortas of apolipoprotein E (Apo-E) knockout (-/-) mice. Methods. Apo-E -/- mice (n = 38) fed a high-cholesterol diet were given SMV (100 mg/kg/day po), RAPA (3 mg/kg/day ip), or no treatment for 10 weeks. Blood was drawn for serum lipid analysis, and protein was extracted for Western immunoblotting. Selected aortic specimens from 2 animals in each group were examined by histology and immunohistochemistry. The data are expressed as the mean ± SEM and compared by the Student t test and ANOVA. Significance was established as P
Databáze: OpenAIRE
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