LPS- and LTA-Induced Expression of IL-6 and TNF-α in Neonatal and Adult Blood: Role of MAPKs and NF-κB
| Autor: | Johannes Poeschl, Kirsten Buschmann, Lutz Koch, David Frommhold, Navina Kuss, Peter Ruef |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Lipopolysaccharides medicine.medical_specialty Article Subject Lipopolysaccharide MAP Kinase Signaling System Pyridines p38 mitogen-activated protein kinases Immunology Stimulation p38 Mitogen-Activated Protein Kinases Monocytes chemistry.chemical_compound Pregnancy Internal medicine medicine lcsh:Pathology Humans Phosphorylation Interleukin 6 Whole blood biology Interleukin-6 Tumor Necrosis Factor-alpha Imidazoles Infant Newborn JNK Mitogen-Activated Protein Kinases NF-kappa B Cell Biology Fetal Blood Flow Cytometry Intracellular signal transduction Teichoic Acids Endocrinology chemistry Gene Expression Regulation biology.protein Tumor necrosis factor alpha Female Lipoteichoic acid Signal Transduction Research Article lcsh:RB1-214 |
| Zdroj: | Mediators of Inflammation, Vol 2014 (2014) Mediators of Inflammation |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | As nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) seem to be critical mediators in the inflammatory response, we studied the effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA) on (a) the activation of NF-κB and MAPKs and (b) the expression of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) with or without the specific inhibitors of these intracellular signal transduction pathways in neonatal cord and adult blood. TNF-αand IL-6 concentrations showed a sharp increase in the supernatants of cord and adult whole blood after stimulation. TNF-αconcentrations were significantly higher, whereas IL-6 concentrations were tendentially lower in adult blood after stimulation. Stimulation with LPS or LTA resulted in a significantly decreased activation of p38 MAPK in neonatal compared with adult blood. Although LTA failed to induce additional ERK1/2 phosphorylation, LPS stimulation mediated the moderately increased levels of activated ERK1/2 in neonatal monocytes. The addition of the p38 MAPK inhibitor SB202190 significantly decreased IL-6 and TNF-αproduction upon LPS or LTA stimulation. Furthermore, the inhibition of ERK1/2 was able to reduce LPS-stimulated TNF-αproduction in neonatal blood. We conclude that p38 MAPK as well as ERK1/2 phosphorylation is crucially involved in LPS activation and could explain the differences in early cytokine response between neonatal and adult blood. |
| Databáze: | OpenAIRE |
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