A novel non-mouse mammary tumor virus activation of the Int-3 gene in a spontaneous mouse mammary tumor
| Autor: | D Gallahan, Gilbert H. Smith, Edith C. Kordon, Robert Callahan |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Gene Expression Regulation
Viral Genes Viral viruses Immunology Molecular Sequence Data Mammary Neoplasms Animal Receptors Cell Surface medicine.disease_cause Microbiology Polymerase Chain Reaction Mice Chimeric RNA Mammary tumor virus Virology Proto-Oncogene Proteins Proto-Oncogenes medicine Animals Promoter Regions Genetic Receptor Notch4 Repetitive Sequences Nucleic Acid Regulation of gene expression Gene Rearrangement Mutation Mammary tumor Hyperplasia biology Base Sequence Receptors Notch Mouse mammary tumor virus Gene rearrangement biology.organism_classification Molecular biology Long terminal repeat Blotting Southern Tumor Virus Infections Mammary Tumor Virus Mouse Insect Science DNA Viral Cancer research Female Retroviridae Infections Research Article |
| Zdroj: | Journal of virology. 69(12) |
| ISSN: | 0022-538X |
| Popis: | In a mouse mammary tumor model system in which carcinogenic progression can be investigated, we have found a unique mutation of Int-3 associated with progression from premalignant lobular hyperplasia to tumor. Sequence analysis of the rearranged fragment revealed an insertion of an intracisternal type A particle (IAP) within the Int-3 gene. Int-3 is mutated frequently in mouse mammary tumor virus (MMTV)-induced mammary tumors by insertion of MMTV proviral DNA into this intragenic region. In these mutations, the insertion produces a chimeric Int-3 transcript encoding the cytoplasmic portion of the Int-3 protein driven by the MMTV long terminal repeat promoter. In this case, the IAP DNA was inserted in the opposite transcriptional orientation relative to Int-3; nevertheless, a similar chimeric RNA transcript driven by a cryptic promoter in the oppositely oriented 5' IAP long terminal repeat was generated. This is the first demonstration that an insertional mutation unrelated to MMTV activates an Int gene commonly associated with mammary tumorigenesis. |
| Databáze: | OpenAIRE |
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