Transformation and mobilization of cloning vectors in Acinetobacter spp
| Autor: | J J van Tuijl, W R Finnerty, J T Singer |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Acinetobacter
biology Genetic Vectors Genetic transfer Cloning vector Molecular cloning biology.organism_classification Microbiology Transformation (genetics) Plasmid Conjugation Genetic Transformation Bacterial Acinetobacter calcoaceticus Cloning Molecular Molecular Biology Bacteria Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 165:301-303 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.165.1.301-303.1986 |
| Popis: | R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 X 10(6) to 6.3 X 10(6) transformants per micrograms of DNA per 10(9) recipient cells. Conjugal mobilization frequencies were 1.1 X 10(-1) to 8.5 X 10(-1) per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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