IGF-1 receptor as an alternative receptor for metabolic signaling in insulin receptor-deficient muscle cells
| Autor: | C. Pinset, Anne Baudry, Rajiv L. Joshi, D. Montarras, Betty Lamothe, Danielle Bucchini, Jacques Jami |
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| Přispěvatelé: | Joshi, Rajiv L., Celogos, Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
MESH: Signal Transduction
[SDV]Life Sciences [q-bio] MESH: Glycogen MESH: Insulin-Like Growth Factor I MyoD Biochemistry MESH: Animals Newborn Culture Media Serum-Free Receptor IGF Type 1 Mice Phosphatidylinositol 3-Kinases MESH: Receptor IGF Type 1 0302 clinical medicine Structural Biology Myocyte Insulin MESH: Animals Insulin-Like Growth Factor I Phosphorylation Receptor Cells Cultured MESH: Muscle Skeletal 0303 health sciences biology Chemistry Myogenesis Metabolic action MESH: Culture Media Serum-Free Cell biology MESH: Glucose [SDV] Life Sciences [q-bio] Signal transduction Mitogen-Activated Protein Kinases Glycogen MESH: Cells Cultured Signal Transduction medicine.medical_specialty MESH: Receptor Insulin Biophysics 030209 endocrinology & metabolism MESH: Insulin Deoxyglucose 03 medical and health sciences Downregulation and upregulation Internal medicine Genetics medicine Animals RNA Messenger Muscle Skeletal MESH: Mice Molecular Biology Myogenin MESH: RNA Messenger 030304 developmental biology Binding Sites MESH: Phosphorylation Muscle cell Cell Biology MESH: Deoxyglucose MESH: Mitogen-Activated Protein Kinases Receptor Insulin Insulin receptor Endocrinology Glucose MESH: Binding Sites Animals Newborn MESH: Gene Deletion MESH: Phosphatidylinositol 3-Kinases MESH: Biomarkers biology.protein Insulin-like growth factor-1 receptor Biomarkers Gene Deletion |
| Zdroj: | FEBS Letters FEBS Letters, Wiley, 2001, 488 (3), pp.174-8 |
| ISSN: | 0014-5793 1873-3468 |
| Popis: | International audience; We have derived skeletal muscle cell lines from wild-type (wt) and insulin receptor (IR) knockout mice to unravel the metabolic potential of IGF-1 receptor (IGF-1R). Both wt and IR(-/-) myoblasts differentiated into myotubes with similar patterns of expression of muscle-specific genes such as MyoD, myogenin and MLC1A indicating that IR is not required for this process. Binding of 125I-IGF-1 on wt and IR(-/-) myotubes was similar showing that IGF-1R was not upregulated in the absence of IR. Stimulation of IR(-/-) myotubes with IGF-1 (10(-10) to 10(-7) M) increased glucose uptake and incorporation into glycogen, induced IRS-1 phosphorylation and activated PI 3-kinase and MAP kinase, two enzymes of major signaling pathways. These effects were comparable to those obtained with wt myotubes using insulin or IGF-1 or with IR(-/-) myotubes using insulin at higher concentrations. This study provides a direct evidence that IGF-1R can represent an alternative receptor for metabolic signaling in muscle cells. |
| Databáze: | OpenAIRE |
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