Heparin-induced multiple electrode aggregometry is a promising and useful functional tool for heparin-induced thrombocytopenia diagnosis: confirmation in a prospective study
| Autor: | Grigoris T. Gerotziafas, Mohamed Hatmi, Francoise Robert, Amir Khaterchi, Vassiliki Galea, Ismail Elalamy |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Oncology
medicine.medical_specialty Platelet aggregation Platelet Aggregation Platelet Function Tests Enzyme-Linked Immunosorbent Assay Platelet Factor 4 Sensitivity and Specificity Antibodies Heparin-induced thrombocytopenia Internal medicine medicine Humans Platelet Prospective Studies Prospective cohort study Adverse effect Whole blood Platelet aggregation test business.industry Heparin Anticoagulants Hematology General Medicine medicine.disease Thrombocytopenia Immunology business medicine.drug |
| Zdroj: | Platelets. 24(6) |
| ISSN: | 1369-1635 |
| Popis: | Heparin-induced thrombocytopenia (HIT) is a potentially lethal adverse effect of heparin therapy. Accurate and rapid HIT laboratory diagnosis when HIT is suspected is crucial. The combination of an immunological assay with a functional test improves the accuracy of HIT, but functional assays are currently limited to a few laboratories. Multiplate® analyzer (Dynabyte, Munich, Germany) is a practical, semi-automated and easy-to-perform platelet aggregation assay. The aim of this study is to explore whether heparin-induced platelet aggregation in whole blood assessed by Multiplate® (Heparin-induced multiple electrode aggregometry, HIMEA) can replace platelet aggregation test (PAT) in platelet-rich plasma. For this purpose, HIMEA performance in HIT diagnosis was prospectively evaluated. HIMEA and PAT were compared to serotonin-release assay (SRA) in 200 well-characterized consecutive patients suspected for HIT. HIMEA was found to be more sensitive (81% vs. 76%) and more specific (99% vs. 96%) than PAT compared to SRA. Both tests showed a high negative predictive value while HIMEA had a better positive predictive value. HIMEA has overall better performance characteristics than PAT for the detection of HIT platelet-activating antibodies. The combination of an immunological assay with HIMEA could be a feasible option in non-specialized laboratories for HIT diagnosis optimization. |
| Databáze: | OpenAIRE |
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