Co-cultures with integrated in situ product removal for lactate-based propionic acid production
| Autor: | Ludwig Selder, Alagappan Lakshmanan, Wael Sabra, An-Ping Zeng, Nikolai Jürgensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
In situ product removal Bioengineering Xylose 01 natural sciences Veillonella 03 medical and health sciences chemistry.chemical_compound Bioreactors 010608 biotechnology Food science Lactic Acid Technik [600] 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Bacillus coagulans 600: Technik Electrodialysis General Medicine biology.organism_classification Bioproduction Propionic acid Coculture Techniques Lactic acid Glucose chemistry Product inhibition Industrial and production engineering Propionates Co-culture ddc:600 Biotechnology Organic acid Research Paper |
| Zdroj: | Bioprocess and Biosystems Engineering Bioprocess and Biosystems Engineering 6 (43): 1027-1035 (2020-06-01) |
| DOI: | 10.15480/882.2763 |
| Popis: | Propionic acid (PA) is a valuable organic acid for the food and feed industry, but no bioproduction at industrial scale exists so far. As product inhibition is a major burden for bioprocesses producing organic acids, in situ product removal (ISPR) is desirable. Here, we demonstrate a new strategy to produce PA with a co-culture coupled with ISPR using electrodialysis. Specifically, Bacillus coagulans first produces lactic acid (LA) from sugar(s) and LA is converted to PA using Veillonella criceti. Applying ISPR to the mentioned co-culture, the specific PA yield was increased from 0.35 to 0.39 g g−1 compared to no ISPR usage. Furthermore, the productivity was increased from 0.63 to 0.7 g L−1 h−1 by applying ISPR. Additionally, it was shown that co-consumption of xylose and glucose led to a higher PA productivity of 0.73 g L−1 h−1, although PA yield was only increased slightly up to 0.36 g g−1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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