Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40
| Autor: | In Geol Choi, Hee Taek Kim, Damao Wang, Kyoung Heon Kim, Jieun Lee, Jae Hyuk Chung, Hee Chul Woo |
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| Rok vydání: | 2011 |
| Předmět: |
Alginates
Gene Expression Uronic acid medicine.disease_cause Polysaccharide Applied Microbiology and Biotechnology Sugar acids Substrate Specificity chemistry.chemical_compound Saccharophagus degradans Enzyme Stability medicine Escherichia coli Cloning Molecular Polysaccharide-Lyases chemistry.chemical_classification biology Depolymerization Alteromonadaceae Temperature Sugar Acids General Medicine Hydrogen-Ion Concentration biology.organism_classification Recombinant Proteins Kinetics Enzyme Biochemistry chemistry Bacteria Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 93(5) |
| ISSN: | 1432-0614 |
| Popis: | Macroalgae are considered to be promising biomass for fuels and chemicals production. To utilize brown macroalgae as biomass, the degradation of alginate, which is the main carbohydrate of brown macroalgae, into monomeric units is a critical prerequisite step. Saccharophagus degradans 2-40 is capable of degrading more than ten different polysaccharides including alginate, and its genome sequence demonstrated that this bacterium contains several putative alginate lyase genes including alg17C. The gene for Alg17C, which is classified into the PL-17 family, was cloned and overexpressed in Escherichia coli. The recombinant Alg17C was found to preferentially act on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-L: -erythro-5-hexoseulose uronic acid. The optimal pH and temperature for Alg17C were found to be 6 and 40 °C, respectively. The K (M) and V (max) of Alg17C were 35.2 mg/ml and 41.7 U/mg, respectively. Based on the results of this study, Alg17C could be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production. |
| Databáze: | OpenAIRE |
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