Lactobacillus caseistrain Shirota protects against nonalcoholic steatohepatitis development in a rodent model
| Autor: | Okubo, H., Sakoda, H., Kushiyama, A., Fujishiro, M., Nakatsu, Y., Fukushima, Toshiaki, Matsunaga, Y., Kamata, H., Asahara, T., Yoshida, Y., Chonan, O., Iwashita, M., Nishimura, F., Asano, T. |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Lactobacillus casei
Lipopolysaccharide Physiology Gut flora digestive system Microbiology Mice chemistry.chemical_compound Methionine Non-alcoholic Fatty Liver Disease Oral administration Fibrosis Physiology (medical) Lactobacillus medicine Animals Gastrointestinal Tract/metabolism/microbiology Choline Deficiency/metabolism Bifidobacterium Inflammation Inflammation/metabolism/pathology Methionine/deficiency/metabolism Liver/metabolism/pathology Hepatology biology Gene Expression Profiling Microbiota Gastroenterology nutritional and metabolic diseases Lipid Metabolism biology.organism_classification medicine.disease Fatty Liver/metabolism/pathology/prevention & control Microbiota/*physiology eye diseases digestive system diseases Choline Deficiency Lactic acid Fatty Liver Gastrointestinal Tract Mice Inbred C57BL Lacticaseibacillus casei Disease Models Animal Bifidobacterium/isolation & purification/metabolism Liver chemistry Lactobacillus casei/*metabolism Gene Expression Profiling/methods |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 305:G911-G918 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00225.2013 |
| Popis: | Gut microbiota alterations are associated with various disorders. In this study, gut microbiota changes were investigated in a methionine-choline-deficient (MCD) diet-induced nonalcoholic steatohepatitis (NASH) rodent model, and the effects of administering Lactobacillus casei strain Shirota (LcS) on the development of NASH were also investigated. Mice were divided into three groups, given the normal chow diet (NCD), MCD diet, or the MCD diet plus daily oral administration of LcS for 6 wk. Gut microbiota analyses for the three groups revealed that lactic acid bacteria such as Bifidobacterium and Lactobacillus in feces were markedly reduced by the MCD diet. Interestingly, oral administration of LcS to MCD diet-fed mice increased not only the L. casei subgroup but also other lactic acid bacteria. Subsequently, NASH development was evaluated based on hepatic histochemical findings, serum parameters, and various mRNA and/or protein expression levels. LcS intervention markedly suppressed MCD-diet-induced NASH development, with reduced serum lipopolysaccharide concentrations, suppression of inflammation and fibrosis in the liver, and reduced colon inflammation. Therefore, reduced populations of lactic acid bacteria in the colon may be involved in the pathogenesis of MCD diet-induced NASH, suggesting normalization of gut microbiota to be effective for treating NASH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|