THE HISTOCHEMICAL DEMONSTRATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN MYOCARDIUM
| Autor: | N. R. Niles, Lucille Bitensky, J. Chayen, G. J. Cunningham |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Adenosine Triphosphatases
chemistry.chemical_classification Adenosine triphosphatase Ion Transport Histology Histocytochemistry Magnesium Myocardium Research chemistry.chemical_element Calcium Phosphate Rats chemistry.chemical_compound Adenosine Triphosphate Enzyme Biochemistry chemistry Myosin Anatomy Adenosine triphosphate |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 12:740-743 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/12.10.740 |
| Popis: | Adenosine triphosphatase has been demonstrated histochemically in rat and human myocardium. To obtain its precise localization in discrete bands, apparently corresponding to the concentration of myosin, it was necessary to modify the existing technique to obtain better preservation of unfixed tissue and maximal enzymatic activity. Thus it was necessary to increase the concentration of calcium and to effect the reaction at pH 9.4 after treatment with 2:4-dinitrophenol. The specificity of the reaction was shown by these factors, by testing with phosphate esters other than adenosine triphosphate, and by the inhibitory effect of magnesium. |
| Databáze: | OpenAIRE |
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