Locating and activating molecular 'time bombs': induction of Mycolata prophages

Autor: Teagan L. Brown, Stephen R. Doyle, Joseph Tucci, Robert J. Seviour, Steve Petrovski, Zoe A. Dyson, Ben Farrar
Rok vydání: 2023
Předmět:
0301 basic medicine
Tsukamurella
Prophages
lcsh:Medicine
Protein Sequencing
medicine.disease_cause
Genome
Nocardia
Mycolic acid
Database and Informatics Methods
Rhodococcus
Bacteriophages
Gordonia Bacterium
lcsh:Science
Uncategorized
chemistry.chemical_classification
Genetics
Viral Genomics
Multidisciplinary
Sewage
Chromosome Mapping
Genomics
Genomic Databases
Gordonia malaquae
Viruses
Sequence Analysis
Research Article
food.ingredient
Sequence Databases
Genome
Viral
Microbial Genomics
Biology
Gordonia
Research and Analysis Methods
Microbiology
Evolution
Molecular
03 medical and health sciences
food
Caudovirales
Sequence Motif Analysis
Virology
Actinomycetales
medicine
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
DNA sequence analysis
Prophage
lcsh:R
Organisms
Biology and Life Sciences
Computational Biology
Genome Analysis
biology.organism_classification
Mutagenesis
Insertional
Biological Databases
030104 developmental biology
chemistry
Virus Activation
lcsh:Q
Zdroj: PLoS ONE, Vol 11, Iss 8, p e0159957 (2016)
PLoS ONE
DOI: 10.26181/22430452.v1
Popis: Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.
Databáze: OpenAIRE
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