Biotransformation of caffeoyl quinic acids from green coffee extracts by Lactobacillus johnsonii NCC 533
| Autor: | Rachid Bel-Rhlid, Dinesh Thapa, Lutz Fischer, Carl Erik Hansen, Karin Kraehenbuehl |
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| Rok vydání: | 2013 |
| Předmět: |
biology
Decarboxylation L. johnsonii Biophysics 4-vinylcatechol Chlorogenic acid biology.organism_classification Esterase Applied Microbiology and Biotechnology Hydrolysis chemistry.chemical_compound chemistry Biotransformation Biochemistry Green coffee extract Decarboxylase Caffeic acid Original Article Lactobacillus johnsonii |
| Zdroj: | AMB Express |
| ISSN: | 2191-0855 |
| Popis: | The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L. johnsonii esterase occurred during the first 16 h of reaction time. No dihydrocaffeic acid was identified in the reaction mixture. The decarboxylation of CA into 4-vinylcatechol (4-VC) started only when the maximum concentration of CA was reached (10 μmol/ml). CA was completely transformed into 4-VC after 48 h of incubation. No 4-vinylphenol or other derivatives could be identified in the reaction media. In this study we demonstrate the capability of L. johnsonii to transform chlorogenic acids from green coffee extract into 4-VC in two steps one pot reaction. Thus, the enzymatic potential of certain lactobacilli might be explored to generate flavor compounds from plant polyphenols. |
| Databáze: | OpenAIRE |
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