Effect of endothelin-1 on neutrophil adhesion to endothelial cells and perfused heart
| Autor: | M J Gallego, Enzo Digiuni, A. Riesco, F Rivas, V Alvarez, A. Lopez Farre, M Montón, Manuela Cernadas, J. Egido, G Espinosa |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Endothelium Neutrophils Macrophage-1 Antigen CD18 Biology In Vitro Techniques Physiology (medical) Integrin complex medicine Cell Adhesion Animals Humans Lymphocyte function-associated antigen 1 Cell adhesion Cells Cultured Peroxidase Cell adhesion molecule Endothelins Heart Neutrophil extracellular traps Molecular biology Lymphocyte Function-Associated Antigen-1 Endothelial stem cell Perfusion medicine.anatomical_structure Immunology Cattle Endothelium Vascular Rabbits Cardiology and Cardiovascular Medicine Cell Adhesion Molecules |
| Zdroj: | Circulation. 88(3) |
| ISSN: | 0009-7322 |
| Popis: | BACKGROUND Based on recent evidence showing that endothelin-1 stimulates several activation mechanisms on neutrophils, the aim of the present study was to analyze the effects of endothelin-1 on neutrophil adhesion to endothelial cells and neutrophil accumulation in the heart. METHODS AND RESULTS The experiments included (1) adhesion of 51Cr-labeled human neutrophils to bovine endothelial cells in culture both in the presence and absence of monoclonal antibodies against the alpha- and beta-subunits of integrins; (2) surface expression of the alpha- and beta-integrin antigens; (3) accumulation of 51Cr-labeled neutrophils on the isolated perfused rabbit heart; (4) in vivo accumulation of autologous neutrophils in the heart, as assessed by myeloperoxidase activity. Endothelin-1 stimulated neutrophil adhesion to endothelial cells (increase of 1 x 10(5) +/- 1 x 10(4) neutrophils per well). The endothelin-1-induced adhesion was blocked (83 +/- 6%) by the anti-CD18 antibody TS1/18 and by several anti-alpha-subunit antibodies. The expression of CD18 and CD11b on the neutrophil surface was also increased by endothelin-1. Endothelin-1 enhanced neutrophil accumulation in the isolated rabbit heart by 4.2 times throughout a TS1/18-inhibitable mechanism. Myeloperoxidase activity increased by 4.2 times in hearts infused in vivo with endothelin-1. CONCLUSIONS Endothelin-1 stimulates neutrophil adhesion to endothelial cells by an effect on the expression of adhesive molecules on the neutrophil surface. Endothelin-1 stimulates neutrophil accumulation in vivo and in vitro in the heart. Antibodies against the integrin complex block the endothelin-1-dependent neutrophil adhesion. These findings have potential importance in the pathophysiology of endothelin-1-increased states. |
| Databáze: | OpenAIRE |
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