Prevention of lymphocytic thyroiditis in iodide-treated non-obese diabetic mice lacking interferon regulatory factor-1
| Autor: | Kouki Mori, Saeko Hoshikawa, Jun Ichi Tani, Katsumi Yoshida, Sadayoshi Ito, Yoshinori Nakagawa, Jo Satoh, Tetsuya Nakazawa |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Population Nod Biology Thyroiditis Mice Endocrinology Mice Inbred NOD Internal medicine Diabetes Mellitus medicine Animals Interferon gamma education NOD mice Mice Knockout education.field_of_study Incidence Thyroiditis Autoimmune General Medicine Iodides Phosphoproteins medicine.disease DNA-Binding Proteins Cytokine CD8 Lymphocytic Thyroiditis Interferon Regulatory Factor-1 medicine.drug |
| Zdroj: | European Journal of Endocrinology. :809-814 |
| ISSN: | 1479-683X 0804-4643 |
| Popis: | OBJECTIVE: Interferon regulatory factor-1 (IRF-1) is a critical regulator of interferon-gamma(IFNgamma)-mediated immune responses. To determine whether IRF-1 is involved in the pathogenesis of thyroiditis in animal models, we evaluated the incidence of iodide-induced lymphocytic thyroiditis (LT) in non-obese diabetic (NOD) mice lacking IRF-1 as well as IRF-1 +/+ and +/- mice. DESIGN: IRF-1 +/+, +/- and -/- NOD mice at 6 weeks of age were fed water (group 1) or iodide water (group 2) for 8 weeks. METHODS: Thyroids were examined histopathologically and intrathyroidal lymphocytic infiltration was arbitrarily graded. Serum thyroxine (T(4)) and anti-mouse thyroglobulin antibody (anti-mTgAb) levels were measured. Spleen cell population was analyzed by flow cytometry, and IFNgamma and interleukin-10 produced by splenocytes were measured by enzyme-linked immunosorbent assay. RESULTS: In group 1, only 4.3% of NOD mice developed LT. In contrast, 67.6% of mice in group 2 developed the disease. Iodide treatment induced LT in more than 80% of IRF-1 +/+ and +/- mice. However, no IRF-1 -/- mice in group 2 developed LT. There was no difference in both serum anti-mTgAb and T(4) levels among the three IRF-1 genotypes of NOD mice. Numbers of splenic CD8(+) T cells and IFNgamma production by Concanavalin A-stimulated splenocytes were markedly decreased in IRF-1-deficient NOD mice. CONCLUSIONS: IRF-1 is involved in the development of iodide-induced LT in NOD mice. |
| Databáze: | OpenAIRE |
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