Cells from Normal and Malignant Human Colon Mucosa Differentially Inhibit Embryonic Cell Aggregation
| Autor: | David E. Maslow, Arnold Mittleman, Eric Mayhew |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Colon Model system Chick Embryo Adenocarcinoma Inhibitory postsynaptic potential Models Biological chemistry.chemical_compound medicine Animals Humans Intestinal Mucosa Cells Cultured Cell Aggregation Retina Retinal General Medicine medicine.disease Embryonic stem cell Cell biology medicine.anatomical_structure Oncology chemistry Colonic Neoplasms Cancer cell Human colon |
| Zdroj: | Oncology. 38:292-294 |
| ISSN: | 1423-0232 0030-2414 |
| DOI: | 10.1159/000225573 |
| Popis: | A model system for studying some aspects of the interaction of cancer cells in tumors and their surrounding nonmalignant tissue is the co-culture of cancer cells and embryonic chick neural retinal cells in gyratory shakers. Neural retina cell aggregation, under these conditions, has been shown to be differentially inhibited by small numbers of cultured mouse and human cancer cells. We extend here these observations to co-cultures of retinal cells with small numbers (60:1 ratio) of human cells isolated from normal colon mucosa or colonic adenocarcinoma tissue. The cells from the malignant tissue samples inhibited aggregation to a significantly greater extent than the cells from normal mucosa, even when both were from the same individual. Cells derived from nonmalignant tumors were more inhibitory than those from normal individuals, which is consistent with described differences in this 'transitional' region. Thus, the aggregation inhibition assay appears applicable to freshly isolated human tissues. |
| Databáze: | OpenAIRE |
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