A novel fluorometric measurement system based on triple complex for mercury (II) determination
| Autor: | İbrahim Ethem Özyiğit, Nergis Arsu, Yavuz Selim Toksöz, Emine Karakuş, Cigdem Bilen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Analytical chemistry
chemistry.chemical_element 02 engineering and technology 010402 general chemistry 01 natural sciences Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Fluorometer Fluorometry Emission spectrum Bovine serum albumin Spectroscopy Instrumentation biology Serum Albumin Bovine Mercury 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Mercury (element) Spectrometry Fluorescence chemistry biology.protein 0210 nano-technology Thioacetic acid |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 248 |
| ISSN: | 1873-3557 |
| Popis: | T-BSA binary complex was formed with non-covalent interactions between fluorometric 2-thioxanthone thioacetic acid and stabilizator bovine serum albumin by fluorescence emission spectroscopy as a sensible and practical method. T-BSA concentration at 1.77 × 10−4 M was obtained as the most suitable and reliable amount for the formation of T-BSA-Hg(II) triple complex. Trace amount of Hg(II) analyses were achieved by this new fluorometric triple complex system as the primary aim. The emission spectra from 350 nm to 650 nm were assayed on fluorometer for Hg(II) concentrations from 1.77 × 10−8 M to 3.53 × 10−4 M under an excitation wavelength of 337 nm. Hg(II) was found to increase the emission intensity of T-BSA by 50% even at 1.77 × 10−7 M Hg(II). So this new system has strong sensitivity to Hg(II) ion. T-BSA-Hg(II) triple complex formation and its fluorometric characterization have not been investigated in literature yet. This study is critically important to provide Hg(II) analyses in wastewater treatments and biological samples for further studies. |
| Databáze: | OpenAIRE |
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