Isolation and structures of grammistins, peptide toxins from the skin secretion of the soapfish Grammistes sexlineatus
| Autor: | Tomoko Igarashi, Yuji Nagashima, Masami Ishida, Kazuo Shiomi, Hiroshi Yokota |
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| Rok vydání: | 2000 |
| Předmět: |
Molecular Sequence Data
Oryzias Venom Peptide Biology In Vitro Techniques Toxicology medicine.disease_cause Hemolysis Melittin Lethal Dose 50 chemistry.chemical_compound Fish Venoms Grammistes sexlineatus medicine Animals Secretion Amino Acid Sequence Horses Chromatography High Pressure Liquid Skin chemistry.chemical_classification Sheep Toxin Circular Dichroism biology.organism_classification Amino acid chemistry Biochemistry Chromatography Gel Cattle Marine Toxins Helical wheel Rabbits Fishes Poisonous |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 38(1) |
| ISSN: | 0041-0101 |
| Popis: | Two peptide toxins (named grammistins Gs 1 and Gs 2) with hemolytic and ichthyotoxic activities were isolated from the skin secretion of the soapfish Grammistes sexlineatus. Grammistin Gs 2 showed 6–11× higher hemolytic activity and 10× higher ichthyotoxicity than grammistin Gs 1. The complete amino acid sequences of Gs 1 comprising 25 residues and Gs 2 comprising 24 residues were determined. Although a search by the database failed to find any homologous toxins from other sources, the grammistins were similar in secondary structures as well as biological activities to the two classes of peptide toxins, melittin from the bee venom and pardaxins from the skin secretion of two species of soles. CD experiments and helical wheel projections showed that the grammistins were randomly coiled in distilled water but formed amphiphilic α-helices in the presence of SDS micelles. In addition, they were found to be surface seeking peptides by the Eisenberg plot and assumed to exist as aggregates of 3–4 molecules. Interestingly, grammistin Gs 2 is much more abundant in amphiphilic α-helices and much higher in biological activities than melittin and pardaxins as well as grammistin Gs 1. |
| Databáze: | OpenAIRE |
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