A genome-wide library of MADM mice for single-cell genetic mosaic analysis
| Autor: | Nicole Amberg, Lill Andersen, Amarbayasgalan Davaatseren, Liqun Luo, Johanna Sonntag, Simon Hippenmeyer, Andi H. Hansen, Thomas Rülicke, Anna-Magdalena Heger, Lindsay A. Schwarz, Carmen Streicher, Tina Bernthaler, Randy L. Johnson, Ximena Contreras |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Genetic Markers Lineage (genetic) Mitosis Somatic stem cell division Mice Transgenic Computational biology Biology Chromatids Genome Models Biological General Biochemistry Genetics and Molecular Biology Sister chromatid segregation Article 03 medical and health sciences Genomic Imprinting 0302 clinical medicine Chromosome Segregation Neoplasms Animals Stem Cell Niche Gene Gene Library Mice Knockout Recombination Genetic Mosaicism Uniparental Disomy Phenotype Chromosomes Mammalian Mice Inbred C57BL Adult Stem Cells Disease Models Animal 030104 developmental biology Adenomatous Polyposis Coli Liver Stem cell Single-Cell Analysis Genomic imprinting 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports Cell Rep |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2021.109274 |
| Popis: | SUMMARY: Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to 96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division. IN BRIEF: Contreras et al. generate a resource and suite of transgenic MADM mice for genetic mosaic analysis with double markers of >96% of the entire mouse genome. In addition to providing a proof of principle, they find non-random mitotic sister chromatid segregation in distinct somatic cell lineages in vivo. |
| Databáze: | OpenAIRE |
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