Pitfalls in Monitoring Mitochondrial Temperature Using Charged Thermosensitive Fluorophores
| Autor: | Paule Bénit, Sunyou Park, Guy Lenaers, Jung Yeol Lee, Young-Tae Chang, Christine Leroy, Malgorzata Rak, Dominique Chretien, Riyad El-Khoury, Pierre Rustin |
|---|---|
| Přispěvatelé: | Maladies neurodéveloppementales et neurovasculaires (NeuroDiderot (UMR_S_1141 / U1141)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neuromuscular Diagnostic Laboratory [Beyrouth, Liban], Department of Pathology & Laboratory Medicine [Beyrouth, Liban], American University of Beirut Medical Center [Beyrouth, Liban] (AUBMC), American University of Beirut [Beyrouth] (AUB)-American University of Beirut [Beyrouth] (AUB)-American University of Beirut Medical Center [Beyrouth, Liban] (AUBMC), American University of Beirut [Beyrouth] (AUB)-American University of Beirut [Beyrouth] (AUB), Department of Chemistry [Pohang, Corée du Sud], Pohang University of Science and Technology (POSTECH), Physiopathologie Cardiovasculaire et Mitochondriale (MITOVASC), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell physiology Cell type [SDV]Life Sciences [q-bio] MitoThermo Yellow Mitochondrion Analytical Chemistry HeLa lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine lcsh:QD415-436 Physical and Theoretical Chemistry ComputingMilieux_MISCELLANEOUS Membrane potential biology Chemistry HEK 293 cells temperature biology.organism_classification Fluorescence mitochondria 030104 developmental biology thermosensitive fluorescent probes Cell culture Biophysics human activities 030217 neurology & neurosurgery |
| Zdroj: | Chemosensors Chemosensors, MDPI, 2020, 8 (4), pp.124. ⟨10.3390/chemosensors8040124⟩ Chemosensors, Vol 8, Iss 124, p 124 (2020) Volume 8 Issue 4 |
| ISSN: | 2227-9040 |
| DOI: | 10.3390/chemosensors8040124⟩ |
| Popis: | Mitochondria are the source of internal heat which influences all cellular processes. Hence, monitoring mitochondrial temperature provides a unique insight into cell physiology. Using a thermosensitive fluorescent probe MitoThermo Yellow (MTY), we have shown recently that mitochondria within human cells are maintained at close to 50 ° C when active, increasing their temperature locally by about 10 ° C. Initially reported in the HEK293 cell line, we confirmed this finding in the HeLa cell line. Delving deeper, using MTY and MTX (MitoThermo X), a modified version of MTY, we unraveled some caveats related to the nature of these charged fluorophores. While enabling the assessment of mitochondrial temperature in HEK and HeLa cell lines, the reactivity of MTY to membrane potential variations in human primary skin fibroblasts precluded local temperature monitoring in these cells. Chemical modification of MTY into MTX did not result in a temperature probe unresponsive to membrane potential variations that could be universally used in any cell type to determine mitochondrial temperature. Thus, the cell-type dependence of MTY in measuring mitochondrial temperature, which is likely due to the variable binding of this dye to specific internal mitochondrial components, should imply cautiousness while using these nanothermometers for mitochondrial temperature analysis. |
| Databáze: | OpenAIRE |
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