Subacute systemic toxicity assessment of β-tricalcium phosphate/carboxymethyl-chitin composite implanted in rat femur
| Autor: | Kazunari Hasegawa, Yusuke Yoshihara, Kazuaki Muramatsu, Shingo Masuda, Kousuke Oba, Daisuke Mukai |
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| Rok vydání: | 2007 |
| Předmět: |
Calcium Phosphates
Male medicine.medical_specialty Pathology Materials science Urinalysis Biomedical Engineering Biophysics Urology Biocompatible Materials Chitin Bioengineering Bone healing Biomaterials chemistry.chemical_compound Implants Experimental Internal medicine Materials Testing medicine Animals Femur Hematology medicine.diagnostic_test Phosphate Rats Radiography chemistry Blood chemistry Bone Substitutes Female Histopathology |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 18:513-522 |
| ISSN: | 1573-4838 0957-4530 |
| Popis: | The efficacy of a composite of beta-tricalcium phosphate particles and carboxymethyl-chitin (beta-TCP/CM-chitin) for bone repair has already been established in animal experiments. In the present study, subacute systemic toxicity was evaluated to further assess the biological safety of the implanted composite. beta-TCP/CM-chitin (approximately 4 mg/kg and 7 mg/kg in male and female rats, respectively) was implanted for 28 days into penetrating defects (2 mm diameter) made artificially in the shaft of the right femur of rats. Sham operation groups with the defect only were prepared as controls. Haematology, blood chemistry, urinalysis, and the histopathology of 44 organs and tissues were investigated. Body weight measurements and clinical observations were performed daily throughout the study. No subacute systemic toxicity possibly caused by the implantation of beta-TCP/CM-chitin was detected. These findings indicate that beta-TCP/CM-chitin composite is a highly biocompatible bone substitute, at least with an implantation dosage of < 4-7 mg/kg. |
| Databáze: | OpenAIRE |
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