Telomere repeats induce domains of H3K27 methylation in Neurospora

Autor: Kirsty Jamieson, Eric U. Selker, Shinji Honda, Tereza Ormsby, Neena A. Leggett, Kevin J McNaught
Rok vydání: 2017
Předmět:
0301 basic medicine
QH301-705.5
Heterochromatin
Science
Methylation
polycomb repressive complex 2
Neurospora
General Biochemistry
Genetics and Molecular Biology
Neurospora crassa
Histones
03 medical and health sciences
Histone H3
position effect
0302 clinical medicine
Gene Expression Regulation
Fungal
Constitutive heterochromatin
Biology (General)
Repetitive Sequences
Nucleic Acid
030304 developmental biology
Genomic organization
Genetics
H3K27
0303 health sciences
General Immunology and Microbiology
biology
General Neuroscience
fungi
N. crassa
General Medicine
Telomere
biology.organism_classification
Subtelomere
chromosome rearrangements
telomere repeats
030104 developmental biology
Position effect
Histone
Genes and Chromosomes
biology.protein
Medicine
Protein Processing
Post-Translational
030217 neurology & neurosurgery
Research Article
Zdroj: eLife, Vol 7 (2018)
eLife
DOI: 10.1101/189258
Popis: Development in higher organisms requires selective gene silencing, directed in part by di-/tri-methylation of lysine 27 on histone H3 (H3K27me2/3). Knowledge of the cues that control formation of such repressive Polycomb domains is extremely limited. We exploited natural and engineered chromosomal rearrangements in the fungusNeurospora crassato elucidate the control of H3K27me2/3. Analyses of H3K27me2/3 in strains bearing chromosomal rearrangements revealed both position-dependent and position-independent facultative heterochromatin. We found that proximity to chromosome ends is necessary to maintain, and sufficient to induce, transcriptionally repressive, subtelomeric H3K27me2/3. We ascertained that such telomere-proximal facultative heterochromatin requires native telomere repeats and found that a short array of ectopic telomere repeats, (TTAGGG)17, can induce a large domain (∼225 kb) of H3K27me2/3. This provides an example of acis-acting sequence that directs H3K27 methylation. Our findings provide new insight into the relationship between genome organization and control of heterochromatin formation.
Databáze: OpenAIRE
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